4ksr

From Proteopedia

(Difference between revisions)

Revision as of 09:11, 7 December 2022

Crystal Structure of the Vibrio cholerae ATPase GspE Hexamer

Structural highlights

4ksr is a 3 chain structure with sequence from Pseudomonas aeruginosa UCBPP-PA14 and Vibrio cholerae O1 biovar El Tor str. N16961. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A0A0H2ZJ24_PSEAB GSPE_VIBCH Involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of proteins. Required for secretion of cholera toxin through the outer membrane.

Publication Abstract from PubMed

The type II secretion system (T2SS), a multiprotein machinery spanning two membranes in Gram-negative bacteria, is responsible for the secretion of folded proteins from the periplasm across the outer membrane. The critical multidomain T2SS assembly ATPase GspEEpsE had not been structurally characterized as a hexamer. Here, four hexamers of Vibrio cholerae GspEEpsE are obtained when fused to Hcp1 as an assistant hexamer, as shown with native mass spectrometry. The enzymatic activity of the GspEEpsE-Hcp1 fusions is approximately 20 times higher than that of a GspEEpsE monomer, indicating that increasing the local concentration of GspEEpsE by the fusion strategy was successful. Crystal structures of GspEEpsE-Hcp1 fusions with different linker lengths reveal regular and elongated hexamers of GspEEpsE with major differences in domain orientation within subunits, and in subunit assembly. SAXS studies on GspEEpsE-Hcp1 fusions suggest that even further variability in GspEEpsE hexamer architecture is likely.

Hexamers of the Type II Secretion ATPase GspE from Vibrio cholerae with Increased ATPase Activity.,Lu C, Turley S, Marionni ST, Park YJ, Lee KK, Patrick M, Shah R, Sandkvist M, Bush MF, Hol WG Structure. 2013 Aug 13. pii: S0969-2126(13)00256-6. doi:, 10.1016/j.str.2013.06.027. PMID:23954505^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lu C, Turley S, Marionni ST, Park YJ, Lee KK, Patrick M, Shah R, Sandkvist M, Bush MF, Hol WG. Hexamers of the Type II Secretion ATPase GspE from Vibrio cholerae with Increased ATPase Activity. Structure. 2013 Aug 13. pii: S0969-2126(13)00256-6. doi:, 10.1016/j.str.2013.06.027. PMID:23954505 doi:10.1016/j.str.2013.06.027

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4ksr"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of the Vibrio cholerae ATPase GspE Hexamer==
-<StructureSection load='4ksr' size='340' side='right' caption='[[4ksr]], [[Resolution|resolution]] 4.20&Aring;' scene=''>
+<StructureSection load='4ksr' size='340' side='right'caption='[[4ksr]], [[Resolution|resolution]] 4.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4ksr]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseab Pseab]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KSR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KSR FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4ksr]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_UCBPP-PA14 Pseudomonas aeruginosa UCBPP-PA14] and [https://en.wikipedia.org/wiki/Vibrio_cholerae_O1_biovar_El_Tor_str._N16961 Vibrio cholerae O1 biovar El Tor str. N16961]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KSR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KSR FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4kss|4kss]]</td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ksr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ksr OCA], [https://pdbe.org/4ksr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ksr RCSB], [https://www.ebi.ac.uk/pdbsum/4ksr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ksr ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">epsE, VC_2732, PA14_01030 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208963 PSEAB])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ksr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ksr OCA], [http://pdbe.org/4ksr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ksr RCSB], [http://www.ebi.ac.uk/pdbsum/4ksr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ksr ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/GSPE_VIBCH GSPE_VIBCH]] Involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of proteins. Required for secretion of cholera toxin through the outer membrane.
+[https://www.uniprot.org/uniprot/A0A0H2ZJ24_PSEAB A0A0H2ZJ24_PSEAB] [https://www.uniprot.org/uniprot/GSPE_VIBCH GSPE_VIBCH] Involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of proteins. Required for secretion of cholera toxin through the outer membrane.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 19: / Line 17: @@
 </div>
 <div class="pdbe-citations 4ksr" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[General secretion pathway protein 3D structures|General secretion pathway protein 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Pseab]]
+[[Category: Large Structures]]
-[[Category: Bush, M]]
+[[Category: Pseudomonas aeruginosa UCBPP-PA14]]
-[[Category: Hol, W G]]
+[[Category: Vibrio cholerae O1 biovar El Tor str. N16961]]
-[[Category: Lee, K]]
+[[Category: Bush M]]
-[[Category: Lu, C Y]]
+[[Category: Hol WG]]
-[[Category: Marionni, S T]]
+[[Category: Lee K]]
-[[Category: Park, Y J]]
+[[Category: Lu CY]]
-[[Category: Patrick, M]]
+[[Category: Marionni ST]]
-[[Category: Sandkvist, M]]
+[[Category: Park YJ]]
-[[Category: Shah, R]]
+[[Category: Patrick M]]
-[[Category: Turley, S]]
+[[Category: Sandkvist M]]
-[[Category: C2 cyclic symmetry]]
+[[Category: Shah R]]
-[[Category: Epse]]
+[[Category: Turley S]]
-[[Category: Hexamer]]
-[[Category: Protein transport]]
-[[Category: T2ss secretion atpase]]

4ksr

From Proteopedia

Revision as of 09:11, 7 December 2022

Crystal Structure of the Vibrio cholerae ATPase GspE Hexamer

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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