|
|
| Line 1: |
Line 1: |
| | | | |
| | ==Structure of U3-55K== | | ==Structure of U3-55K== |
| - | <StructureSection load='4j0w' size='340' side='right' caption='[[4j0w]], [[Resolution|resolution]] 1.70Å' scene=''> | + | <StructureSection load='4j0w' size='340' side='right'caption='[[4j0w]], [[Resolution|resolution]] 1.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4j0w]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J0W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J0W FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4j0w]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J0W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4J0W FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4j0x|4j0x]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4j0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j0w OCA], [https://pdbe.org/4j0w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4j0w RCSB], [https://www.ebi.ac.uk/pdbsum/4j0w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4j0w ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RRP9, RNU3IP2, U355K ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j0w OCA], [http://pdbe.org/4j0w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4j0w RCSB], [http://www.ebi.ac.uk/pdbsum/4j0w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4j0w ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/U3IP2_HUMAN U3IP2_HUMAN]] Component of a nucleolar small nuclear ribonucleoprotein particle (snoRNP) thought to participate in the processing and modification of pre-ribosomal RNA. | + | [https://www.uniprot.org/uniprot/U3IP2_HUMAN U3IP2_HUMAN] Component of a nucleolar small nuclear ribonucleoprotein particle (snoRNP) thought to participate in the processing and modification of pre-ribosomal RNA. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 23: |
Line 21: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Lin, J]] | + | [[Category: Large Structures]] |
| - | [[Category: Ye, K]] | + | [[Category: Lin J]] |
| - | [[Category: Zhang, L]] | + | [[Category: Ye K]] |
| - | [[Category: Beta-propeller]] | + | [[Category: Zhang L]] |
| - | [[Category: Preribosome]]
| + | |
| - | [[Category: Rna binding protein]]
| + | |
| - | [[Category: Wd domain]]
| + | |
| Structural highlights
Function
U3IP2_HUMAN Component of a nucleolar small nuclear ribonucleoprotein particle (snoRNP) thought to participate in the processing and modification of pre-ribosomal RNA.
Publication Abstract from PubMed
The U3 snoRNA is required for 18S rRNA processing and small subunit ribosome formation in eukaryotes. Different from other box C/D snoRNAs, U3 contains an extra 5' domain that pairs with pre-rRNA and a unique B/C motif essential for recruitment of the U3-specific Rrp9 protein. Here, we analyze the structure and function of Rrp9 with crystallographic, biochemical, and cellular approaches. Rrp9 is composed of a WD repeat domain and an N-terminal region. The crystal structures of the WD domain of yeast Rrp9 and its human ortholog U3-55K were determined, revealing a typical seven-bladed propeller fold. Several conserved surface patches on the WD domain were identified, and their function in RNP assembly and yeast growth were analyzed by mutagenesis. Prior association of Snu13 with the B/C motif was found to enhance the specific binding of the WD domain. We show that a conserved 7bc loop is crucial for specific recognition of U3, nucleolar localization of Rrp9, and yeast growth. In addition, we show that the N-terminal region of Rrp9 contains a bipartite nuclear localization signal that is dispensable for nucleolar localization. Our results provide insight into the functional sites of Rrp9.
Structural and functional analysis of the U3 snoRNA binding protein Rrp9.,Zhang L, Lin J, Ye K RNA. 2013 May;19(5):701-11. doi: 10.1261/rna.037580.112. Epub 2013 Mar 18. PMID:23509373[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Zhang L, Lin J, Ye K. Structural and functional analysis of the U3 snoRNA binding protein Rrp9. RNA. 2013 May;19(5):701-11. doi: 10.1261/rna.037580.112. Epub 2013 Mar 18. PMID:23509373 doi:10.1261/rna.037580.112
|
