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| | ==Crystal Structure of MTIP from Plasmodium falciparum in complex with HBS myoA, a hydrogen bond surrogate myoA helix mimetic== | | ==Crystal Structure of MTIP from Plasmodium falciparum in complex with HBS myoA, a hydrogen bond surrogate myoA helix mimetic== |
| - | <StructureSection load='4mzl' size='340' side='right' caption='[[4mzl]], [[Resolution|resolution]] 2.01Å' scene=''> | + | <StructureSection load='4mzl' size='340' side='right'caption='[[4mzl]], [[Resolution|resolution]] 2.01Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4mzl]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Plaf7 Plaf7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MZL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MZL FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4mzl]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum_3D7 Plasmodium falciparum 3D7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MZL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MZL FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=2JC:N-ETHYLGLYCINE'>2JC</scene>, <scene name='pdbligand=BUA:BUTANOIC+ACID'>BUA</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2JC:N-ETHYLGLYCINE'>2JC</scene>, <scene name='pdbligand=BUA:BUTANOIC+ACID'>BUA</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4aom|4aom]], [[4mzk|4mzk]], [[4mzj|4mzj]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mzl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mzl OCA], [https://pdbe.org/4mzl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mzl RCSB], [https://www.ebi.ac.uk/pdbsum/4mzl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mzl ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MTIP, PFL2225w ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36329 PLAF7])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mzl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mzl OCA], [http://pdbe.org/4mzl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mzl RCSB], [http://www.ebi.ac.uk/pdbsum/4mzl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4mzl ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MYOA_PLAF7 MYOA_PLAF7]] Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments (By similarity). | + | [https://www.uniprot.org/uniprot/Q8I4W8_PLAF7 Q8I4W8_PLAF7] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Plaf7]] | + | [[Category: Large Structures]] |
| - | [[Category: Cota, E]] | + | [[Category: Plasmodium falciparum 3D7]] |
| - | [[Category: Douse, C H]] | + | [[Category: Cota E]] |
| - | [[Category: Garnett, J A]]
| + | [[Category: Douse CH]] |
| - | [[Category: Maas, S J]]
| + | [[Category: Garnett JA]] |
| - | [[Category: Tate, E W]]
| + | [[Category: Maas SJ]] |
| - | [[Category: Actomyosin motor]] | + | [[Category: Tate EW]] |
| - | [[Category: Alpha-helix mimetic]] | + | |
| - | [[Category: Protein binding-inhibitor complex]] | + | |
| - | [[Category: Stapled peptide]] | + | |
| Structural highlights
Function
Q8I4W8_PLAF7
Publication Abstract from PubMed
Constrained alpha-helical peptides are an exciting class of molecule designed to disrupt protein-protein interactions (PPIs) at a surface-exposed helix binding site. Complexes that engage more than one helical face account for over a third of structurally characterized helix PPIs, including several examples where the helix is fully buried. However, no constrained peptides have been reported that have targeted this class of interaction. We report the design of stapled and hydrogen bond surrogate (HBS) peptides mimicking the helical tail of the malaria parasite invasion motor myosin (myoA), which presents polar and hydrophobic functionality on all three faces in binding its partner, myoA tail interacting protein (MTIP), with high affinity. The first structures of these different constrained peptides bound to the same target are reported, enabling a direct comparison between these constraints and between staples based on monosubstituted pentenyl glycine (pGly) and disubstituted pentenyl alanine (pAla). Importantly, installation of these constraints does not disrupt native interactions in the buried site, so the affinity of the wild-type peptide is maintained.
Crystal Structures of Stapled and Hydrogen Bond Surrogate Peptides Targeting a Fully Buried Protein-Helix Interaction.,Douse CH, Maas SJ, Thomas JC, Garnett JA, Sun Y, Cota E, Tate EW ACS Chem Biol. 2014 Aug 6. PMID:25084543[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Douse CH, Maas SJ, Thomas JC, Garnett JA, Sun Y, Cota E, Tate EW. Crystal Structures of Stapled and Hydrogen Bond Surrogate Peptides Targeting a Fully Buried Protein-Helix Interaction. ACS Chem Biol. 2014 Aug 6. PMID:25084543 doi:http://dx.doi.org/10.1021/cb500271c
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