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| | ==Crystal structure of the cytosolic domain of mouse MiD51 dimer mutant== | | ==Crystal structure of the cytosolic domain of mouse MiD51 dimer mutant== |
| - | <StructureSection load='4oai' size='340' side='right' caption='[[4oai]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='4oai' size='340' side='right'caption='[[4oai]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4oai]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OAI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OAI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4oai]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OAI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OAI FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4oaf|4oaf]], [[4oag|4oag]], [[4aoh|4aoh]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4oai FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oai OCA], [https://pdbe.org/4oai PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4oai RCSB], [https://www.ebi.ac.uk/pdbsum/4oai PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4oai ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Mid51, MiD51 (aka SMCR7L), Mief1, Smcr7l ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oai FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oai OCA], [http://pdbe.org/4oai PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4oai RCSB], [http://www.ebi.ac.uk/pdbsum/4oai PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4oai ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MID51_MOUSE MID51_MOUSE]] Mitochondrial outer membrane protein which regulates mitochondrial fission. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface independently of the mitochondrial fission FIS1 and MFF proteins. | + | [https://www.uniprot.org/uniprot/MID51_MOUSE MID51_MOUSE] Mitochondrial outer membrane protein which regulates mitochondrial fission. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface independently of the mitochondrial fission FIS1 and MFF proteins. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Large Structures]] |
| - | [[Category: Chan, D C]] | + | [[Category: Mus musculus]] |
| - | [[Category: Kaiser, J T]] | + | [[Category: Chan DC]] |
| - | [[Category: Loson, O C]] | + | [[Category: Kaiser JT]] |
| - | [[Category: Nucleotidyl transferase fold]] | + | [[Category: Loson OC]] |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
MID51_MOUSE Mitochondrial outer membrane protein which regulates mitochondrial fission. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface independently of the mitochondrial fission FIS1 and MFF proteins.
Publication Abstract from PubMed
Mitochondrial fission requires recruitment of dynamin-related protein 1 (Drp1) to the mitochondrial surface and activation of its GTP-dependent scission function. The Drp1 receptors MiD49 and MiD51 recruit Drp1 to facilitate mitochondrial fission, but their mechanism of action is poorly understood. Using X-ray crystallography, we demonstrate that MiD51 contains a nucleotidyl transferase domain that binds ADP with high affinity. MiD51 recruits Drp1 via a surface loop that functions independently of ADP binding. However, in the absence of nucleotide binding, the recruited Drp1 cannot be activated for fission. Purified MiD51 strongly inhibits Drp1 assembly and GTP hydrolysis in the absence of ADP. Addition of ADP relieves this inhibition and promotes Drp1 assembly into spirals with enhanced GTP hydrolysis. Our results reveal ADP as an essential cofactor for MiD51 during mitochondrial fission.
The Mitochondrial Fission Receptor MiD51 Requires ADP as a Cofactor.,Loson OC, Liu R, Rome ME, Meng S, Kaiser JT, Shan SO, Chan DC Structure. 2014 Mar 4;22(3):367-77. doi: 10.1016/j.str.2014.01.001. Epub 2014 Feb, 6. PMID:24508339[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Loson OC, Liu R, Rome ME, Meng S, Kaiser JT, Shan SO, Chan DC. The Mitochondrial Fission Receptor MiD51 Requires ADP as a Cofactor. Structure. 2014 Mar 4;22(3):367-77. doi: 10.1016/j.str.2014.01.001. Epub 2014 Feb, 6. PMID:24508339 doi:http://dx.doi.org/10.1016/j.str.2014.01.001
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