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3rfh

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Revision as of 06:01, 15 June 2022

Crystal structure of the yeast RACK1 dimer in space group P21

Structural highlights

3rfh is a 4 chain structure with sequence from Baker's yeast. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	3rfg, 3frx, 3dm0, 1trj
Gene:	ASC1, CPC2, YM9718.15C, YMR116C (Baker's yeast)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[GBLP_YEAST] Located at the head of the 40S ribosomal subunit in the vicinity of the mRNA exit channel, it serves as a scaffold protein that can recruit other proteins to the ribosome. Involved in the negative regulation of translation of a specific subset of proteins.^[1]

Publication Abstract from PubMed

Receptor for activated C-kinase 1 (RACK1) serves as a scaffolding protein in numerous signaling pathways involving kinases and membrane-bound receptors from different cellular compartments. It exists simultaneously as a cytosolic free form and as a ribosome-bound protein. As part of the 40S ribosomal subunit, it triggers translational regulation by establishing a direct link between protein kinase C and the protein synthesis machinery. It has been suggested that RACK1 could recruit other signaling molecules onto the ribosome, providing a signal-specific modulation of the translational process. RACK1 is able to dimerize both in vitro and in vivo. This homodimer formation has been observed in several processes including the regulation of the N-methyl-d-aspartate receptor by the Fyn kinase in the brain and the oxygen-independent degradation of hypoxia-inducible factor 1. The functional relevance of this dimerization is, however, still unclear and the question of a possible dimerization of the ribosome-bound protein is still pending. Here, we report the first structure of a RACK1 homodimer, as determined from two independent crystal forms of the Saccharomyces cerevisiae RACK1 protein (also known as Asc1p) at 2.9 and 3.9 A resolution. The structure reveals an atypical mode of dimerization where monomers intertwine on blade 4, thus exposing a novel surface of the protein to potential interacting partners. We discuss the significance of the dimer structure for RACK1 function.

Structure of the RACK1 dimer from Saccharomyces cerevisiae.,Yatime L, Hein KL, Nilsson J, Nissen P J Mol Biol. 2011 Aug 12;411(2):486-98. Epub 2011 Jun 17. PMID:21704636^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Gerbasi VR, Weaver CM, Hill S, Friedman DB, Link AJ. Yeast Asc1p and mammalian RACK1 are functionally orthologous core 40S ribosomal proteins that repress gene expression. Mol Cell Biol. 2004 Sep;24(18):8276-87. PMID:15340087 doi:10.1128/MCB.24.18.8276-8287.2004
↑ Yatime L, Hein KL, Nilsson J, Nissen P. Structure of the RACK1 dimer from Saccharomyces cerevisiae. J Mol Biol. 2011 Aug 12;411(2):486-98. Epub 2011 Jun 17. PMID:21704636 doi:10.1016/j.jmb.2011.06.017

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3rfh"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the yeast RACK1 dimer in space group P21==
-<StructureSection load='3rfh' size='340' side='right' caption='[[3rfh]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
+<StructureSection load='3rfh' size='340' side='right'caption='[[3rfh]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3rfh]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RFH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RFH FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3rfh]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RFH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RFH FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3rfg|3rfg]], [[3frx|3frx]], [[3dm0|3dm0]], [[1trj|1trj]]</td></tr>
+</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3rfg|3rfg]], [[3frx|3frx]], [[3dm0|3dm0]], [[1trj|1trj]]</div></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ASC1, CPC2, YM9718.15C, YMR116C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ASC1, CPC2, YM9718.15C, YMR116C ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rfh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rfh OCA], [http://pdbe.org/3rfh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3rfh RCSB], [http://www.ebi.ac.uk/pdbsum/3rfh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3rfh ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rfh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rfh OCA], [https://pdbe.org/3rfh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rfh RCSB], [https://www.ebi.ac.uk/pdbsum/3rfh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rfh ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/GBLP_YEAST GBLP_YEAST]] Located at the head of the 40S ribosomal subunit in the vicinity of the mRNA exit channel, it serves as a scaffold protein that can recruit other proteins to the ribosome. Involved in the negative regulation of translation of a specific subset of proteins.<ref>PMID:15340087</ref>
+[[https://www.uniprot.org/uniprot/GBLP_YEAST GBLP_YEAST]] Located at the head of the 40S ribosomal subunit in the vicinity of the mRNA exit channel, it serves as a scaffold protein that can recruit other proteins to the ribosome. Involved in the negative regulation of translation of a specific subset of proteins.<ref>PMID:15340087</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 21: @@
 ==See Also==
-*[[Receptor for activated protein kinase C 1|Receptor for activated protein kinase C 1]]
+*[[3D sructureseceptor for activated protein kinase C 1|3D sructureseceptor for activated protein kinase C 1]]
 == References ==
 <references/>
@@ Line 27: / Line 27: @@
 </StructureSection>
 [[Category: Baker's yeast]]
+[[Category: Large Structures]]
 [[Category: Hein, K L]]
 [[Category: Nilsson, J]]

3rfh

From Proteopedia

Revision as of 06:01, 15 June 2022

Crystal structure of the yeast RACK1 dimer in space group P21

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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