1lb1
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1lb1.jpg|left|200px]] | [[Image:1lb1.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1lb1", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_1lb1| PDB=1lb1 | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Crystal Structure of the Dbl and Pleckstrin homology domains of Dbs in complex with RhoA''' | '''Crystal Structure of the Dbl and Pleckstrin homology domains of Dbs in complex with RhoA''' | ||
| Line 34: | Line 31: | ||
[[Category: Sondek, J.]] | [[Category: Sondek, J.]] | ||
[[Category: Worthylake, D K.]] | [[Category: Worthylake, D K.]] | ||
| - | [[Category: | + | [[Category: Db]] |
| - | [[Category: | + | [[Category: Dh domain]] |
| - | [[Category: | + | [[Category: Guanine nucleotide exchange factor]] |
| - | [[Category: | + | [[Category: Ph domain]] |
| - | [[Category: | + | [[Category: Rhoa]] |
| - | [[Category: | + | [[Category: Small g-protein]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:44:20 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 20:44, 2 May 2008
Crystal Structure of the Dbl and Pleckstrin homology domains of Dbs in complex with RhoA
Overview
Activation of Rho-family GTPases involves the removal of bound GDP and the subsequent loading of GTP, all catalyzed by guanine nucleotide exchange factors (GEFs) of the Dbl-family. Despite high sequence conservation among Rho GTPases, Dbl proteins possess a wide spectrum of discriminatory potentials for Rho-family members. To rationalize this specificity, we have determined crystal structures of the conserved, catalytic fragments (Dbl and pleckstrin homology domains) of the exchange factors intersectin and Dbs in complex with their cognate GTPases, Cdc42 and RhoA, respectively. Structure-based mutagenesis of intersectin and Dbs reveals the key determinants responsible for promoting exchange activity in Cdc42, Rac1 and RhoA. These findings provide critical insight into the structural features necessary for the proper pairing of Dbl-exchange factors with Rho GTPases and now allow for the detailed manipulation of signaling pathways mediated by these oncoproteins in vivo.
About this Structure
1LB1 is a Protein complex structure of sequences from Homo sapiens and Mus musculus. Full crystallographic information is available from OCA.
Reference
Structural basis for the selective activation of Rho GTPases by Dbl exchange factors., Snyder JT, Worthylake DK, Rossman KL, Betts L, Pruitt WM, Siderovski DP, Der CJ, Sondek J, Nat Struct Biol. 2002 Jun;9(6):468-75. PMID:12006984 Page seeded by OCA on Fri May 2 23:44:20 2008
