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| | ==Structure of the minimal Ste5 VWA domain subject to autoinhibition by the Ste5 PH domain== | | ==Structure of the minimal Ste5 VWA domain subject to autoinhibition by the Ste5 PH domain== |
| - | <StructureSection load='4f2h' size='340' side='right' caption='[[4f2h]], [[Resolution|resolution]] 3.19Å' scene=''> | + | <StructureSection load='4f2h' size='340' side='right'caption='[[4f2h]], [[Resolution|resolution]] 3.19Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4f2h]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F2H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F2H FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4f2h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F2H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F2H FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">STE5, NUL3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f2h OCA], [https://pdbe.org/4f2h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f2h RCSB], [https://www.ebi.ac.uk/pdbsum/4f2h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f2h ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f2h OCA], [http://pdbe.org/4f2h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4f2h RCSB], [http://www.ebi.ac.uk/pdbsum/4f2h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4f2h ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/STE5_YEAST STE5_YEAST]] Component of the pheromone signal transduction pathway. It mediates pheromone signals acting between STE20 and STE11. It is absolutely required for pheromone-induced transcription of FUS1. May play a role in cell-cycle arrest in response to pheromone. | + | [https://www.uniprot.org/uniprot/STE5_YEAST STE5_YEAST] Component of the pheromone signal transduction pathway. It mediates pheromone signals acting between STE20 and STE11. It is absolutely required for pheromone-induced transcription of FUS1. May play a role in cell-cycle arrest in response to pheromone. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 18824]] | + | [[Category: Large Structures]] |
| - | [[Category: Coyle, S M]] | + | [[Category: Saccharomyces cerevisiae]] |
| - | [[Category: Lim, W A]] | + | [[Category: Coyle SM]] |
| - | [[Category: Zalatan, J G]] | + | [[Category: Lim WA]] |
| - | [[Category: Coactivation of fus3]] | + | [[Category: Zalatan JG]] |
| - | [[Category: Signaling protein]]
| + | |
| - | [[Category: Ste5m]]
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| - | [[Category: Von wildebrand type some]]
| + | |
| Structural highlights
Function
STE5_YEAST Component of the pheromone signal transduction pathway. It mediates pheromone signals acting between STE20 and STE11. It is absolutely required for pheromone-induced transcription of FUS1. May play a role in cell-cycle arrest in response to pheromone.
Publication Abstract from PubMed
Cells reuse signaling proteins in multiple pathways, raising the potential for improper crosstalk. Scaffold proteins are thought to insulate against such miscommunication by sequestering proteins into distinct physical complexes. We show that the scaffold protein Ste5, which organizes the yeast mating mitogen-activated protein (MAP) kinase pathway, does not use sequestration to prevent misactivation of the mating response. Instead, Ste5 appears to use a conformation mechanism: Under basal conditions, intramolecular interaction of the PH domain with the VWA domain blocks the ability to coactivate the mating-specific MAPK, Fus3. Pheromone-induced membrane binding of Ste5 triggers release of this autoinhibition. Thus, in addition to serving as a conduit guiding kinase communication, Ste5 directly receives input information to decide if and when signal can be transmitted to mating output.
Conformational Control of the Ste5 Scaffold Protein Insulates Against MAP Kinase Misactivation.,Zalatan JG, Coyle SM, Rajan S, Sidhu SS, Lim WA Science. 2012 Aug 9. PMID:22878499[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Zalatan JG, Coyle SM, Rajan S, Sidhu SS, Lim WA. Conformational Control of the Ste5 Scaffold Protein Insulates Against MAP Kinase Misactivation. Science. 2012 Aug 9. PMID:22878499 doi:10.1126/science.1220683
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