1ldz
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1ldz.gif|left|200px]] | [[Image:1ldz.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1ldz", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_1ldz| PDB=1ldz | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''SOLUTION STRUCTURE OF THE LEAD-DEPENDENT RIBOZYME, NMR, 25 STRUCTURES''' | '''SOLUTION STRUCTURE OF THE LEAD-DEPENDENT RIBOZYME, NMR, 25 STRUCTURES''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LDZ OCA]. | |
==Reference== | ==Reference== | ||
NMR solution structure of the lead-dependent ribozyme: evidence for dynamics in RNA catalysis., Hoogstraten CG, Legault P, Pardi A, J Mol Biol. 1998 Nov 27;284(2):337-50. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9813122 9813122] | NMR solution structure of the lead-dependent ribozyme: evidence for dynamics in RNA catalysis., Hoogstraten CG, Legault P, Pardi A, J Mol Biol. 1998 Nov 27;284(2):337-50. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9813122 9813122] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Hoogstraten, C G.]] | [[Category: Hoogstraten, C G.]] | ||
[[Category: Legault, P.]] | [[Category: Legault, P.]] | ||
[[Category: Pardi, A.]] | [[Category: Pardi, A.]] | ||
| - | [[Category: | + | [[Category: Catalytic rna]] |
| - | [[Category: | + | [[Category: Internal loop]] |
| - | [[Category: | + | [[Category: Leadzyme]] |
| - | [[Category: | + | [[Category: Nmr spectroscopy]] |
| - | [[Category: | + | [[Category: Rna structure]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:49:49 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 20:49, 2 May 2008
SOLUTION STRUCTURE OF THE LEAD-DEPENDENT RIBOZYME, NMR, 25 STRUCTURES
Overview
The NMR solution structure of a lead-dependent ribozyme, known as the leadzyme, is presented. This ribozyme is among the smallest of the known catalytic RNAs, with an active site consisting of a six-nucleotide asymmetric internal loop. This loop has a roughly double-helical structure, including a protonated adenine-cytosine wobble base-pair, that positions the cytosine base 5' to the cleavage site in a double-helical conformation. The deviations from helical structure consist of two bulged guanosine residues, G7 and G9, where G7 is the residue 3' to the cleavage site. The scissile phosphate group of the leadzyme is not positioned for in-line nucleophilic attack. Therefore, a conformational rearrangement in the active site is required to reach the proposed transition state for this ribozyme. This is similar to previous observations in X-ray studies of the hammerhead ribozyme, and emphasizes the necessity for dynamic structural fluctuations in the catalytic mechanism of small ribozymes. A model for metal-binding in the leadzyme is proposed in which a lead ion binds to a bulged guanine base that is critical for leadzyme function.
About this Structure
Full crystallographic information is available from OCA.
Reference
NMR solution structure of the lead-dependent ribozyme: evidence for dynamics in RNA catalysis., Hoogstraten CG, Legault P, Pardi A, J Mol Biol. 1998 Nov 27;284(2):337-50. PMID:9813122 Page seeded by OCA on Fri May 2 23:49:49 2008
