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3s6x

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Revision as of 10:40, 22 June 2022

Structure of reovirus attachment protein sigma1 in complex with alpha-2,3-sialyllactose

Structural highlights

3s6x is a 3 chain structure with sequence from Mammalian orthoreovirus 3 strain dearing. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
NonStd Res:
Related:	2oj5, 1kke, 3eoy, 3s6y, 3s6z
Gene:	S1 (Mammalian Orthoreovirus 3 strain Dearing)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SIGM1_REOVD] Fiber-like molecule that attaches the virion to the host cell membrane by binding to the primary receptor F11R/JAM-A and to sialic acid containing proteins (coreceptor). The interaction of sigma-1 with F11R is required for NF-kB activation and apoptosis. Binding to both sialic acid and F11R is required to induce maximal levels of apoptosis.

Publication Abstract from PubMed

Many viruses attach to target cells by binding to cell-surface glycans. To gain a better understanding of strategies used by viruses to engage carbohydrate receptors, we determined the crystal structures of reovirus attachment protein sigma1 in complex with alpha-2,3-sialyllactose, alpha-2,6-sialyllactose, and alpha-2,8-di-siallylactose. All three oligosaccharides terminate in sialic acid, which serves as a receptor for the reovirus serotype studied here. The overall structure of sigma1 resembles an elongated, filamentous trimer. It contains a globular head featuring a compact beta-barrel, and a fibrous extension formed by seven repeating units of a triple beta-spiral that is interrupted near its midpoint by a short alpha-helical coiled coil. The carbohydrate-binding site is located between beta-spiral repeats two and three, distal from the head. In all three complexes, the terminal sialic acid forms almost all of the contacts with sigma1 in an identical manner, while the remaining components of the oligosaccharides make little or no contacts. We used this structural information to guide mutagenesis studies to identify residues in sigma1 that functionally engage sialic acid by assessing hemagglutination capacity and growth in murine erythroleukemia cells, which require sialic acid binding for productive infection. Our studies using sigma1 mutant viruses reveal that residues 198, 202, 203, 204, and 205 are required for functional binding to sialic acid by reovirus. These findings provide insight into mechanisms of reovirus attachment to cell-surface glycans and contribute to an understanding of carbohydrate binding by viruses. They also establish a filamentous, trimeric carbohydrate-binding module that could potentially be used to endow other trimeric proteins with carbohydrate-binding properties.

Crystal structure of reovirus attachment protein sigma1 in complex with sialylated oligosaccharides.,Reiter DM, Frierson JM, Halvorson EE, Kobayashi T, Dermody TS, Stehle T PLoS Pathog. 2011 Aug;7(8):e1002166. Epub 2011 Aug 4. PMID:21829363^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Reiter DM, Frierson JM, Halvorson EE, Kobayashi T, Dermody TS, Stehle T. Crystal structure of reovirus attachment protein sigma1 in complex with sialylated oligosaccharides. PLoS Pathog. 2011 Aug;7(8):e1002166. Epub 2011 Aug 4. PMID:21829363 doi:10.1371/journal.ppat.1002166

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3s6x"

@@ Line 1: / Line 1: @@
 ==Structure of reovirus attachment protein sigma1 in complex with alpha-2,3-sialyllactose==
-<StructureSection load='3s6x' size='340' side='right' caption='[[3s6x]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
+<StructureSection load='3s6x' size='340' side='right'caption='[[3s6x]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3s6x]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mammalian_orthoreovirus_3_strain_dearing Mammalian orthoreovirus 3 strain dearing]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S6X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3S6X FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3s6x]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mammalian_orthoreovirus_3_strain_dearing Mammalian orthoreovirus 3 strain dearing]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S6X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3S6X FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2oj5|2oj5]], [[1kke|1kke]], [[3eoy|3eoy]], [[3s6y|3s6y]], [[3s6z|3s6z]]</td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2oj5|2oj5]], [[1kke|1kke]], [[3eoy|3eoy]], [[3s6y|3s6y]], [[3s6z|3s6z]]</div></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">S1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10886 Mammalian Orthoreovirus 3 strain Dearing])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">S1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10886 Mammalian Orthoreovirus 3 strain Dearing])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3s6x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s6x OCA], [http://pdbe.org/3s6x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3s6x RCSB], [http://www.ebi.ac.uk/pdbsum/3s6x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3s6x ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3s6x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s6x OCA], [https://pdbe.org/3s6x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3s6x RCSB], [https://www.ebi.ac.uk/pdbsum/3s6x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3s6x ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/SIGM1_REOVD SIGM1_REOVD]] Fiber-like molecule that attaches the virion to the host cell membrane by binding to the primary receptor F11R/JAM-A and to sialic acid containing proteins (coreceptor). The interaction of sigma-1 with F11R is required for NF-kB activation and apoptosis. Binding to both sialic acid and F11R is required to induce maximal levels of apoptosis.
+[[https://www.uniprot.org/uniprot/SIGM1_REOVD SIGM1_REOVD]] Fiber-like molecule that attaches the virion to the host cell membrane by binding to the primary receptor F11R/JAM-A and to sialic acid containing proteins (coreceptor). The interaction of sigma-1 with F11R is required for NF-kB activation and apoptosis. Binding to both sialic acid and F11R is required to induce maximal levels of apoptosis.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 21: @@
 </div>
 <div class="pdbe-citations 3s6x" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Mammalian orthoreovirus 3 strain dearing]]
 [[Category: Dermody, T S]]

3s6x

From Proteopedia

Revision as of 10:40, 22 June 2022

Structure of reovirus attachment protein sigma1 in complex with alpha-2,3-sialyllactose

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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