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2wq5

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Revision as of 08:09, 3 August 2017

Non-antibiotic properties of tetracyclines: structural basis for inhibition of secretory phospholipase A2.

Structural highlights

2wq5 is a 1 chain structure with sequence from Naja naja. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Related:	1psh, 1ows, 1a3f, 1a3d
Activity:	Phospholipase A(2), with EC number 3.1.1.4
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[PA2A2_NAJNA] PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Secretory phospholipase A(2) is involved in inflammatory processes and was previously shown to be inhibited by lipophilic tetracyclines such as minocycline (minoTc) and doxycycline. Lipophilic tetracyclines might be a new lead compound for the design of specific inhibitors of secretory phospholipase A(2), which play a crucial role in inflammatory processes. Our X-ray crystal structure analysis at 1.65 A resolution of the minoTc complex of phospholipase A(2) (PLA(2)) of the Indian cobra (Naja naja naja) is the first example of nonantibiotic tetracycline interactions with a protein. MinoTc interferes with the conformation of the active-site Ca(2+)-binding loop, preventing Ca(2)(+) binding, and shields the active site from substrate entrance, resulting in inhibition of the enzyme. MinoTc binding to PLA(2) is dominated by hydrophobic interactions quite different from antibiotic recognition of tetracyclines by proteins or the ribosome. The affinity of minoTc for PLA(2) was determined by surface plasmon resonance, resulting in a dissociation constant K(d)=1.8 x 10(-)(4) M.

Nonantibiotic properties of tetracyclines: structural basis for inhibition of secretory phospholipase A2.,Dalm D, Palm GJ, Aleksandrov A, Simonson T, Hinrichs W J Mol Biol. 2010 Apr 23;398(1):83-96. Epub 2010 Mar 6. PMID:20211188^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Dalm D, Palm GJ, Aleksandrov A, Simonson T, Hinrichs W. Nonantibiotic properties of tetracyclines: structural basis for inhibition of secretory phospholipase A2. J Mol Biol. 2010 Apr 23;398(1):83-96. Epub 2010 Mar 6. PMID:20211188 doi:10.1016/j.jmb.2010.02.049

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2wq5"

@@ Line 1: / Line 1: @@
-==NON-ANTIBIOTIC PROPERTIES OF TETRACYCLINES: STRUCTURAL BASIS FOR INHIBITION OF SECRETORY PHOSPHOLIPASE A2.==
+==Non-antibiotic properties of tetracyclines: structural basis for inhibition of secretory phospholipase A2.==
 <StructureSection load='2wq5' size='340' side='right' caption='[[2wq5]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2wq5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Indian_cobra Indian cobra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WQ5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WQ5 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2wq5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Naja_naja Naja naja]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WQ5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WQ5 FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MIY:(4S,4AS,5AR,12AS)-4,7-BIS(DIMETHYLAMINO)-3,10,12,12A-TETRAHYDROXY-1,11-DIOXO-1,4,4A,5,5A,6,11,12A-OCTAHYDROTETRACENE-2-CARBOXAMIDE'>MIY</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1psh|1psh]], [[1ows|1ows]], [[1a3f|1a3f]], [[1a3d|1a3d]]</td></tr>
@@ Line 37: / Line 37: @@
 __TOC__
 </StructureSection>
-[[Category: Indian cobra]]
+[[Category: Naja naja]]
 [[Category: Dalm, D]]
 [[Category: Hinrichs, W]]

2wq5

From Proteopedia

Revision as of 08:09, 3 August 2017

Non-antibiotic properties of tetracyclines: structural basis for inhibition of secretory phospholipase A2.

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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