3wek
From Proteopedia
(Difference between revisions)
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==Crystal structure of the human squalene synthase F288L mutant in complex with presqualene pyrophosphate== | ==Crystal structure of the human squalene synthase F288L mutant in complex with presqualene pyrophosphate== | ||
| - | <StructureSection load='3wek' size='340' side='right' caption='[[3wek]], [[Resolution|resolution]] 1.85Å' scene=''> | + | <StructureSection load='3wek' size='340' side='right'caption='[[3wek]], [[Resolution|resolution]] 1.85Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3wek]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WEK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WEK FirstGlance]. <br> | <table><tr><td colspan='2'>[[3wek]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WEK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WEK FirstGlance]. <br> | ||
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wek FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wek OCA], [http://pdbe.org/3wek PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wek RCSB], [http://www.ebi.ac.uk/pdbsum/3wek PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wek ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wek FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wek OCA], [http://pdbe.org/3wek PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wek RCSB], [http://www.ebi.ac.uk/pdbsum/3wek PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wek ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Squalene synthase (SQS) is a divalent metal-ion-dependent enzyme that catalyzes the two-step reductive `head-to-head' condensation of two molecules of farnesyl pyrophosphate to form squalene using presqualene diphosphate (PSPP) as an intermediate. In this paper, the structures of human SQS and its mutants in complex with several substrate analogues and intermediates coordinated with Mg2+ or Mn2+ are presented, which stepwise delineate the biosynthetic pathway. Extensive study of the SQS active site has identified several critical residues that are involved in binding reduced nicotinamide dinucleotide phosphate (NADPH). Based on mutagenesis data and a locally closed (JK loop-in) structure observed in the hSQS-(F288L)-PSPP complex, an NADPH-binding model is proposed for SQS. The results identified four major steps (substrate binding, condensation, intermediate formation and translocation) of the ordered sequential mechanisms involved in the `1'-1' isoprenoid biosynthetic pathway. These new findings clarify previous hypotheses based on site-directed mutagenesis and biochemical analysis. | ||
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| + | Structural insights into the catalytic mechanism of human squalene synthase.,Liu CI, Jeng WY, Chang WJ, Shih MF, Ko TP, Wang AH Acta Crystallogr D Biol Crystallogr. 2014 Feb;70(Pt 2):231-41. doi:, 10.1107/S1399004713026230. Epub 2014 Jan 17. PMID:24531458<ref>PMID:24531458</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 3wek" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
*[[Squalene synthase|Squalene synthase]] | *[[Squalene synthase|Squalene synthase]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Human]] | [[Category: Human]] | ||
| + | [[Category: Large Structures]] | ||
[[Category: Squalene synthase]] | [[Category: Squalene synthase]] | ||
[[Category: Jeng, W Y]] | [[Category: Jeng, W Y]] | ||
Revision as of 09:56, 25 December 2019
Crystal structure of the human squalene synthase F288L mutant in complex with presqualene pyrophosphate
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