|
|
| Line 1: |
Line 1: |
| | | | |
| | ==Crystal structure of yeast Mmf1== | | ==Crystal structure of yeast Mmf1== |
| - | <StructureSection load='3quw' size='340' side='right' caption='[[3quw]], [[Resolution|resolution]] 1.75Å' scene=''> | + | <StructureSection load='3quw' size='340' side='right'caption='[[3quw]], [[Resolution|resolution]] 1.75Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3quw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QUW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QUW FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3quw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QUW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QUW FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3qv0|3qv0]]</td></tr> | + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3qv0|3qv0]]</div></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">IBM1, MMF1, YIL051C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr> | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">IBM1, MMF1, YIL051C ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3quw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3quw OCA], [http://pdbe.org/3quw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3quw RCSB], [http://www.ebi.ac.uk/pdbsum/3quw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3quw ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3quw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3quw OCA], [https://pdbe.org/3quw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3quw RCSB], [https://www.ebi.ac.uk/pdbsum/3quw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3quw ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MMF1_YEAST MMF1_YEAST]] Plays a role in the maintenance of mitochondrial DNA. | + | [[https://www.uniprot.org/uniprot/MMF1_YEAST MMF1_YEAST]] Plays a role in the maintenance of mitochondrial DNA. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 24: |
Line 24: |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Atcc 18824]] | | [[Category: Atcc 18824]] |
| | + | [[Category: Large Structures]] |
| | [[Category: Chen, Y]] | | [[Category: Chen, Y]] |
| | [[Category: Jiang, Y L]] | | [[Category: Jiang, Y L]] |
| Structural highlights
Function
[MMF1_YEAST] Plays a role in the maintenance of mitochondrial DNA.
Publication Abstract from PubMed
The yeast Saccharomyces cerevisiae mitochondrial matrix factor Mmf1, a member in the YER057c/Yigf/Uk114 family, participates in isoleucine biosynthesis and mitochondria maintenance. Mmf1 physically interacts with another mitochondrial matrix protein Mam33, which is involved in the sorting of cytochrome b(2) to the intermembrane space as well as mitochondrial ribosomal protein synthesis. To elucidate the structural basis for their interaction, we determined the crystal structures of Mmf1 and Mam33 at 1.74 and 2.10A, respectively. Both Mmf1 and Mam33 adopt a trimeric structure: each subunit of Mmf1 displays a chorismate mutase fold with a six-stranded beta-sheet flanked by two alpha-helices on one side, whereas a subunit of Mam33 consists of a twisted six-stranded beta-sheet surrounded by five alpha-helices. Biochemical assays combined with structure-based computational simulation enable us to model a putative complex of Mmf1-Mam33, which consists of one Mam33 trimer and two tandem Mmf1 trimers in a head-to-tail manner. The two interfaces between the ring-like trimers are mainly composed of electrostatic interactions mediated by complementary negatively and positively charged patches. These results provided the structural insights into the putative function of Mmf1 during mitochondrial protein synthesis via Mam33, a protein binding to mitochondrial ribosomal proteins.
Crystal structures and putative interface of Saccharomyces cerevisiae mitochondrial matrix proteins Mmf1 and Mam33.,Pu YG, Jiang YL, Ye XD, Ma XX, Guo PC, Lian FM, Teng YB, Chen Y, Zhou CZ J Struct Biol. 2011 May 12. PMID:21600990[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Pu YG, Jiang YL, Ye XD, Ma XX, Guo PC, Lian FM, Teng YB, Chen Y, Zhou CZ. Crystal structures and putative interface of Saccharomyces cerevisiae mitochondrial matrix proteins Mmf1 and Mam33. J Struct Biol. 2011 May 12. PMID:21600990 doi:10.1016/j.jsb.2011.05.005
|
