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| | ==Crystal structure of the pED208 TraM N-terminal domain== | | ==Crystal structure of the pED208 TraM N-terminal domain== |
| - | <StructureSection load='3omy' size='340' side='right' caption='[[3omy]], [[Resolution|resolution]] 1.30Å' scene=''> | + | <StructureSection load='3omy' size='340' side='right'caption='[[3omy]], [[Resolution|resolution]] 1.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3omy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OMY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OMY FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3omy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OMY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OMY FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">traM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr> | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">traM ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3omy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3omy OCA], [http://pdbe.org/3omy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3omy RCSB], [http://www.ebi.ac.uk/pdbsum/3omy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3omy ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3omy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3omy OCA], [https://pdbe.org/3omy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3omy RCSB], [https://www.ebi.ac.uk/pdbsum/3omy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3omy ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TRAM8_ECOLX TRAM8_ECOLX]] Conjugative DNA transfer (CDT) is the unidirectional transfer of ssDNA plasmid from a donor to a recipient cell. It is the central mechanism by which antibiotic resistance and virulence factors are propagated in bacterial populations. Part of the relaxosome, which facilitates a site- and strand-specific cut in the origin of transfer by TraI, at the nic site (By similarity). TraM binds to three principal regions in the oriT (transfer origin) region; 2 are required for autoregulation while the other is required for plasmid transfer. Plasmid specificity is conferred by the TraD-TraM pair. | + | [[https://www.uniprot.org/uniprot/TRAM8_ECOLX TRAM8_ECOLX]] Conjugative DNA transfer (CDT) is the unidirectional transfer of ssDNA plasmid from a donor to a recipient cell. It is the central mechanism by which antibiotic resistance and virulence factors are propagated in bacterial populations. Part of the relaxosome, which facilitates a site- and strand-specific cut in the origin of transfer by TraI, at the nic site (By similarity). TraM binds to three principal regions in the oriT (transfer origin) region; 2 are required for autoregulation while the other is required for plasmid transfer. Plasmid specificity is conferred by the TraD-TraM pair. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Bacillus coli migula 1895]] | | [[Category: Bacillus coli migula 1895]] |
| | + | [[Category: Large Structures]] |
| | [[Category: Edwards, R A]] | | [[Category: Edwards, R A]] |
| | [[Category: Frost, L S]] | | [[Category: Frost, L S]] |
| Structural highlights
Function
[TRAM8_ECOLX] Conjugative DNA transfer (CDT) is the unidirectional transfer of ssDNA plasmid from a donor to a recipient cell. It is the central mechanism by which antibiotic resistance and virulence factors are propagated in bacterial populations. Part of the relaxosome, which facilitates a site- and strand-specific cut in the origin of transfer by TraI, at the nic site (By similarity). TraM binds to three principal regions in the oriT (transfer origin) region; 2 are required for autoregulation while the other is required for plasmid transfer. Plasmid specificity is conferred by the TraD-TraM pair.
Publication Abstract from PubMed
The conjugative transfer of F-like plasmids such as F, R1, R100 and pED208, between bacterial cells requires TraM, a plasmid-encoded DNA-binding protein. TraM tetramers bridge the origin of transfer (oriT) to a key component of the conjugative pore, the coupling protein TraD. Here we show that TraM recognizes a high-affinity DNA-binding site, sbmA, as a cooperative dimer of tetramers. The crystal structure of the TraM-sbmA complex from the plasmid pED208 shows that binding cooperativity is mediated by DNA kinking and unwinding, without any direct contact between tetramers. Sequence-specific DNA recognition is carried out by TraM's N-terminal ribbon-helix-helix (RHH) domains, which bind DNA in a staggered arrangement. We demonstrate that both DNA-binding specificity, as well as selective interactions between TraM and the C-terminal tail of its cognate TraD mediate conjugation specificity within the F-like family of plasmids. The ability of TraM to cooperatively bind DNA without interaction between tetramers leaves the C-terminal TraM tetramerization domains free to make multiple interactions with TraD, driving recruitment of the plasmid to the conjugative pore.
Structural basis of cooperative DNA recognition by the plasmid conjugation factor, TraM.,Wong JJ, Lu J, Edwards RA, Frost LS, Glover JN Nucleic Acids Res. 2011 May 11. PMID:21565799[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Wong JJ, Lu J, Edwards RA, Frost LS, Glover JN. Structural basis of cooperative DNA recognition by the plasmid conjugation factor, TraM. Nucleic Acids Res. 2011 May 11. PMID:21565799 doi:10.1093/nar/gkr296
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