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3wxm

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Revision as of 09:18, 21 February 2019

Crystal structure of archaeal Pelota and GTP-bound EF1 alpha complex

Structural highlights

3wxm is a 8 chain structure with sequence from Aerpe. This structure supersedes the now removed PDB entry 3agj. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Gene:	tuf, APE_1844 (AERPE), pelA, APE_1800.1 (AERPE)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[EF1A_AERPE] This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis (By similarity). [PELO_AERPE] May function in recognizing stalled ribosomes, interact with stem-loop structures in stalled mRNA molecules, and effect endonucleolytic cleavage of the mRNA. May play a role in the release non-functional ribosomes and degradation of damaged mRNAs. Has endoribonuclease activity (By similarity).

Publication Abstract from PubMed

No-go decay and nonstop decay are mRNA surveillance pathways that detect translational stalling and degrade the underlying mRNA, allowing the correct translation of the genetic code. In eukaryotes, the protein complex of Pelota (yeast Dom34) and Hbs1 translational GTPase recognizes the stalled ribosome containing the defective mRNA. Recently, we found that archaeal Pelota (aPelota) associates with archaeal elongation factor 1alpha (aEF1alpha) to act in the mRNA surveillance pathway, which accounts for the lack of an Hbs1 ortholog in archaea. Here we present the complex structure of aPelota and GTP-bound aEF1alpha determined at 2.3-A resolution. The structure reveals how GTP-bound aEF1alpha recognizes aPelota and how aPelota in turn stabilizes the GTP form of aEF1alpha. Combined with the functional analysis in yeast, the present results provide structural insights into the molecular interaction between eukaryotic Pelota and Hbs1. Strikingly, the aPelota.aEF1alpha complex structurally resembles the tRNA.EF-Tu complex bound to the ribosome. Our findings suggest that the molecular mimicry of tRNA in the distorted "A/T state" conformation by Pelota enables the complex to efficiently detect and enter the empty A site of the stalled ribosome.

Structural basis for mRNA surveillance by archaeal Pelota and GTP-bound EF1alpha complex.,Kobayashi K, Kikuno I, Kuroha K, Saito K, Ito K, Ishitani R, Inada T, Nureki O Proc Natl Acad Sci U S A. 2010 Oct 12;107(41):17575-9. Epub 2010 Sep 27. PMID:20876129^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kobayashi K, Kikuno I, Kuroha K, Saito K, Ito K, Ishitani R, Inada T, Nureki O. Structural basis for mRNA surveillance by archaeal Pelota and GTP-bound EF1alpha complex. Proc Natl Acad Sci U S A. 2010 Oct 12;107(41):17575-9. Epub 2010 Sep 27. PMID:20876129 doi:http://dx.doi.org/10.1073/pnas.1009598107

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3wxm"

@@ Line 3: / Line 3: @@
 <StructureSection load='3wxm' size='340' side='right' caption='[[3wxm]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3wxm]] is a 8 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3agj 3agj]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WXM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WXM FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3wxm]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Aerpe Aerpe]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3agj 3agj]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WXM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WXM FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">tuf, APE_1844 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272557 AERPE]), pelA, APE_1800.1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272557 AERPE])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wxm OCA], [http://pdbe.org/3wxm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wxm RCSB], [http://www.ebi.ac.uk/pdbsum/3wxm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wxm ProSAT]</span></td></tr>
 </table>
@@ Line 25: / Line 26: @@
 __TOC__
 </StructureSection>
+[[Category: Aerpe]]
 [[Category: Ishitani, R]]
 [[Category: Kobayashi, K]]

3wxm

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Revision as of 09:18, 21 February 2019

Crystal structure of archaeal Pelota and GTP-bound EF1 alpha complex

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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