1ls2
From Proteopedia
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'''Fitting of EF-Tu and tRNA in the Low Resolution Cryo-EM Map of an EF-Tu Ternary Complex (GDP and Kirromycin) Bound to E. coli 70S Ribosome''' | '''Fitting of EF-Tu and tRNA in the Low Resolution Cryo-EM Map of an EF-Tu Ternary Complex (GDP and Kirromycin) Bound to E. coli 70S Ribosome''' | ||
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[[Category: Valle, M.]] | [[Category: Valle, M.]] | ||
[[Category: 70s e coli ribosome]] | [[Category: 70s e coli ribosome]] | ||
| - | [[Category: | + | [[Category: Cryo-em]] |
| - | [[Category: | + | [[Category: Ef-tu]] |
| - | [[Category: | + | [[Category: Ternary complex]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:13:42 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 21:13, 2 May 2008
Fitting of EF-Tu and tRNA in the Low Resolution Cryo-EM Map of an EF-Tu Ternary Complex (GDP and Kirromycin) Bound to E. coli 70S Ribosome
Overview
During the elongation cycle of protein biosynthesis, the specific amino acid coded for by the mRNA is delivered by a complex that is comprised of the cognate aminoacyl-tRNA, elongation factor Tu and GTP. As this ternary complex binds to the ribosome, the anticodon end of the tRNA reaches the decoding center in the 30S subunit. Here we present the cryo- electron microscopy (EM) study of an Escherichia coli 70S ribosome-bound ternary complex stalled with an antibiotic, kirromycin. In the cryo-EM map the anticodon arm of the tRNA presents a new conformation that appears to facilitate the initial codon-anticodon interaction. Furthermore, the elbow region of the tRNA is seen to contact the GTPase-associated center on the 50S subunit of the ribosome, suggesting an active role of the tRNA in the transmission of the signal prompting the GTP hydrolysis upon codon recognition.
About this Structure
1LS2 is a Single protein structure of sequence from Escherichia coli and Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Cryo-EM reveals an active role for aminoacyl-tRNA in the accommodation process., Valle M, Sengupta J, Swami NK, Grassucci RA, Burkhardt N, Nierhaus KH, Agrawal RK, Frank J, EMBO J. 2002 Jul 1;21(13):3557-67. PMID:12093756 Page seeded by OCA on Sat May 3 00:13:42 2008
