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4kny

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Revision as of 09:03, 7 December 2022

Crystal structure of the response regulator KdpE complexed to DNA in an active-like conformation

Structural highlights

4kny is a 4 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

KDPE_ECOLI Member of the two-component regulatory system KdpD/KdpE involved in the regulation of the kdp operon.

Publication Abstract from PubMed

Two-component signal transduction systems consist of pairs of histidine kinases and response regulators, which mediate adaptive responses to environmental cues. Most activated response regulators regulate transcription by binding tightly to promoter DNA via a phosphorylation-triggered inactive-to-active transition. The molecular basis for formation of stable response regulator-DNA complexes that precede the assembly of RNA polymerases is unclear. Here, we present structures of DNA complexed with the response regulator KdpE, a member of the OmpR/PhoB family. The distinctively asymmetric complex in an active-like conformation reveals a unique intramolecular interface between the receiver domain (RD) and the DNA-binding domain (DBD) of only one of the two response regulators in the complex. Structure-function studies show that this RD-DBD interface is necessary to form stable complexes that support gene expression. The conservation of sequence and structure suggests that these findings extend to a large group of response regulators that act as transcription factors.

An asymmetric heterodomain interface stabilizes a response regulator-DNA complex.,Narayanan A, Kumar S, Evrard AN, Paul LN, Yernool DA Nat Commun. 2014 Feb 14;5:3282. doi: 10.1038/ncomms4282. PMID:24526190^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Narayanan A, Kumar S, Evrard AN, Paul LN, Yernool DA. An asymmetric heterodomain interface stabilizes a response regulator-DNA complex. Nat Commun. 2014 Feb 14;5:3282. doi: 10.1038/ncomms4282. PMID:24526190 doi:http://dx.doi.org/10.1038/ncomms4282

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4kny"

Categories: Escherichia coli K-12 | Large Structures | Kumar S | Narayanan A | Yernool DA

@@ Line 1: / Line 1: @@
 ==Crystal structure of the response regulator KdpE complexed to DNA in an active-like conformation==
-<StructureSection load='4kny' size='340' side='right' caption='[[4kny]], [[Resolution|resolution]] 2.94&Aring;' scene=''>
+<StructureSection load='4kny' size='340' side='right'caption='[[4kny]], [[Resolution|resolution]] 2.94&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4kny]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KNY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KNY FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4kny]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KNY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KNY FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">b0694, JW5096, kdpE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kny FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kny OCA], [https://pdbe.org/4kny PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kny RCSB], [https://www.ebi.ac.uk/pdbsum/4kny PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kny ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kny FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kny OCA], [http://pdbe.org/4kny PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4kny RCSB], [http://www.ebi.ac.uk/pdbsum/4kny PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4kny ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/KDPE_ECOLI KDPE_ECOLI]] Member of the two-component regulatory system KdpD/KdpE involved in the regulation of the kdp operon.
+[https://www.uniprot.org/uniprot/KDPE_ECOLI KDPE_ECOLI] Member of the two-component regulatory system KdpD/KdpE involved in the regulation of the kdp operon.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 21: @@
 __TOC__
 </StructureSection>
-[[Category: Ecoli]]
+[[Category: Escherichia coli K-12]]
-[[Category: Kumar, S]]
+[[Category: Large Structures]]
-[[Category: Narayanan, A]]
+[[Category: Kumar S]]
-[[Category: Yernool, D A]]
+[[Category: Narayanan A]]
-[[Category: Dna-binding domain]]
+[[Category: Yernool DA]]
-[[Category: Receiver domain]]
-[[Category: Transcription regulator-dna complex]]

4kny

From Proteopedia

Revision as of 09:03, 7 December 2022

Crystal structure of the response regulator KdpE complexed to DNA in an active-like conformation

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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