This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1lum
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1lum.jpg|left|200px]] | [[Image:1lum.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1lum", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | | | + | --> |
| - | | | + | {{STRUCTURE_1lum| PDB=1lum | SCENE= }} |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''NMR Structure of the Itk SH2 domain, Pro287trans, 20 low energy structures''' | '''NMR Structure of the Itk SH2 domain, Pro287trans, 20 low energy structures''' | ||
| Line 30: | Line 27: | ||
[[Category: Fulton, D B.]] | [[Category: Fulton, D B.]] | ||
[[Category: Mallis, R J.]] | [[Category: Mallis, R J.]] | ||
| - | [[Category: | + | [[Category: Cis/trans isomerization]] |
| - | [[Category: | + | [[Category: Interleukin-2 tyrosine kinase]] |
| - | [[Category: | + | [[Category: Itk]] |
| - | [[Category: | + | [[Category: Proline]] |
| - | [[Category: | + | [[Category: Sh2]] |
| - | [[Category: | + | [[Category: Src homology 2]] |
| - | [[Category: | + | [[Category: T-cell specific kinase]] |
| - | [[Category: | + | [[Category: Tsk]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:18:30 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 21:18, 2 May 2008
NMR Structure of the Itk SH2 domain, Pro287trans, 20 low energy structures
Overview
Interleukin-2 tyrosine kinase (Itk) is a T cell-specific kinase required for a proper immune response following T cell receptor engagement. In addition to the kinase domain, Itk is composed of several noncatalytic regulatory domains, including a Src homology 2 (SH2) domain that contains a conformationally heterogeneous Pro residue. Cis-trans isomerization of a single prolyl imide bond within the SH2 domain mediates conformer-specific ligand recognition that may have functional implications in T cell signaling. To better understand the mechanism by which a proline switch regulates ligand binding, we have used NMR spectroscopy to determine two structures of Itk SH2 corresponding to the cis and trans imide bond-containing conformers. The structures indicate that the heterogeneous Pro residue acts as a hinge that modulates ligand recognition by controlling the relative orientation of protein-binding surfaces.
About this Structure
1LUM is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Structural characterization of a proline-driven conformational switch within the Itk SH2 domain., Mallis RJ, Brazin KN, Fulton DB, Andreotti AH, Nat Struct Biol. 2002 Dec;9(12):900-5. PMID:12402030 Page seeded by OCA on Sat May 3 00:18:30 2008
