This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1lwy
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1lwy.gif|left|200px]] | [[Image:1lwy.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1lwy", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_1lwy| PDB=1lwy | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''hOgg1 Borohydride-Trapped Intermediate without 8-oxoguanine''' | '''hOgg1 Borohydride-Trapped Intermediate without 8-oxoguanine''' | ||
| Line 30: | Line 27: | ||
[[Category: Verdine, G L.]] | [[Category: Verdine, G L.]] | ||
[[Category: Yang, W.]] | [[Category: Yang, W.]] | ||
| - | [[Category: | + | [[Category: Borohydride]] |
| - | [[Category: | + | [[Category: Covalent trapping]] |
| - | [[Category: | + | [[Category: Dna glycosylase]] |
| - | [[Category: | + | [[Category: Dna repair]] |
| - | [[Category: | + | [[Category: Product-assisted catalysis]] |
| - | [[Category: | + | [[Category: Protein/dna]] |
| - | [[Category: | + | [[Category: Reaction intermediate]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:23:10 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 21:23, 2 May 2008
hOgg1 Borohydride-Trapped Intermediate without 8-oxoguanine
Overview
Most spontaneous damage to bases in DNA is corrected through the action of the base-excision DNA repair pathway. Base excision repair is initiated by DNA glycosylases, lesion-specific enzymes that intercept aberrant bases in DNA and catalyze their excision. How such proteins accomplish the feat of catalyzing no fewer than five sequential reaction steps using a single active site has been unknown. To help answer this, we report the structure of a trapped catalytic intermediate in DNA repair by human 8-oxoguanine DNA glycosylase. This structure and supporting biochemical results reveal that the enzyme sequesters the excised lesion base and exploits it as a cofactor to participate in catalysis. To our knowledge, the present example represents the first documented case of product-assisted catalysis in an enzyme-catalyzed reaction.
About this Structure
1LWY is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Product-assisted catalysis in base-excision DNA repair., Fromme JC, Bruner SD, Yang W, Karplus M, Verdine GL, Nat Struct Biol. 2003 Mar;10(3):204-11. PMID:12592398 Page seeded by OCA on Sat May 3 00:23:10 2008
