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| | ==Nucleoporin ELYS (aa1-494), Mus musculus== | | ==Nucleoporin ELYS (aa1-494), Mus musculus== |
| - | <StructureSection load='4i0o' size='340' side='right' caption='[[4i0o]], [[Resolution|resolution]] 1.90Å' scene=''> | + | <StructureSection load='4i0o' size='340' side='right'caption='[[4i0o]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4i0o]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4I0O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4I0O FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4i0o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4I0O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4I0O FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Ahctf1, Elys ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4i0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4i0o OCA], [https://pdbe.org/4i0o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4i0o RCSB], [https://www.ebi.ac.uk/pdbsum/4i0o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4i0o ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4i0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4i0o OCA], [http://pdbe.org/4i0o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4i0o RCSB], [http://www.ebi.ac.uk/pdbsum/4i0o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4i0o ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ELYS_MOUSE ELYS_MOUSE]] Required for the assembly of a functional nuclear pore complex (NPC) on the surface of chromosomes as nuclei form at the end of mitosis. May initiate NPC assembly by binding to chromatin and recruiting the Nup107-160 subcomplex of the NPC. Also required for the localization of the Nup107-160 subcomplex of the NPC to the kinetochore during mitosis and for the completion of cytokinesis (By similarity). Has also been proposed to function as a transcription factor which may play a specific role in hematopoietic tissues (PubMed:11952839).<ref>PMID:11952839</ref> | + | [https://www.uniprot.org/uniprot/ELYS_MOUSE ELYS_MOUSE] Required for the assembly of a functional nuclear pore complex (NPC) on the surface of chromosomes as nuclei form at the end of mitosis. May initiate NPC assembly by binding to chromatin and recruiting the Nup107-160 subcomplex of the NPC. Also required for the localization of the Nup107-160 subcomplex of the NPC to the kinetochore during mitosis and for the completion of cytokinesis (By similarity). Has also been proposed to function as a transcription factor which may play a specific role in hematopoietic tissues (PubMed:11952839).<ref>PMID:11952839</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Large Structures]] |
| - | [[Category: Bilokapic, S]] | + | [[Category: Mus musculus]] |
| - | [[Category: Schwartz, T U]] | + | [[Category: Bilokapic S]] |
| - | [[Category: Beta propeller]] | + | [[Category: Schwartz TU]] |
| - | [[Category: Mrna transport]]
| + | |
| - | [[Category: Nuclear pore complex]]
| + | |
| - | [[Category: Structural protein]]
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| - | [[Category: Wd40 repeat]]
| + | |
| Structural highlights
Function
ELYS_MOUSE Required for the assembly of a functional nuclear pore complex (NPC) on the surface of chromosomes as nuclei form at the end of mitosis. May initiate NPC assembly by binding to chromatin and recruiting the Nup107-160 subcomplex of the NPC. Also required for the localization of the Nup107-160 subcomplex of the NPC to the kinetochore during mitosis and for the completion of cytokinesis (By similarity). Has also been proposed to function as a transcription factor which may play a specific role in hematopoietic tissues (PubMed:11952839).[1]
Publication Abstract from PubMed
In metazoa, the nuclear envelope (NE), together with the embedded nuclear pore complexes (NPCs), breaks down and reassembles during cell division. It is suggested that ELYS, a nucleoporin, binds to chromatin in an initial step of postmitotic NPC assembly and subsequently recruits the essential Y-subcomplex, the major scaffolding unit of the NPC. Here, we show that ELYS contains three domains: an N-terminal beta-propeller domain, a central alpha-helical domain, and a C-terminal disordered region. While the disordered region is responsible for the interactions with chromatin, the two preceding domains synergistically mediate tethering to the NPC. We present the crystal structure of the seven-bladed beta-propeller domain at 1.9 A resolution. Analysis of the beta-propeller surface reveals the regions that are required for NPC anchorage. We discuss the possible roles of ELYS in the context of the NPC scaffold architecture.
Structural and Functional Studies of the 252 kDa Nucleoporin ELYS Reveal Distinct Roles for Its Three Tethered Domains.,Bilokapic S, Schwartz TU Structure. 2013 Apr 2;21(4):572-80. doi: 10.1016/j.str.2013.02.006. Epub 2013 Mar, 14. PMID:23499022[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kimura N, Takizawa M, Okita K, Natori O, Igarashi K, Ueno M, Nakashima K, Nobuhisa I, Taga T. Identification of a novel transcription factor, ELYS, expressed predominantly in mouse foetal haematopoietic tissues. Genes Cells. 2002 Apr;7(4):435-46. PMID:11952839
- ↑ Bilokapic S, Schwartz TU. Structural and Functional Studies of the 252 kDa Nucleoporin ELYS Reveal Distinct Roles for Its Three Tethered Domains. Structure. 2013 Apr 2;21(4):572-80. doi: 10.1016/j.str.2013.02.006. Epub 2013 Mar, 14. PMID:23499022 doi:http://dx.doi.org/10.1016/j.str.2013.02.006
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