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| | ==Crystal structure of Deg5== | | ==Crystal structure of Deg5== |
| - | <StructureSection load='4ic5' size='340' side='right' caption='[[4ic5]], [[Resolution|resolution]] 2.61Å' scene=''> | + | <StructureSection load='4ic5' size='340' side='right'caption='[[4ic5]], [[Resolution|resolution]] 2.61Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4ic5]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IC5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IC5 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4ic5]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IC5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IC5 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DEGP5, HHOA, At4g18370, F28J12.30 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ic5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ic5 OCA], [https://pdbe.org/4ic5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ic5 RCSB], [https://www.ebi.ac.uk/pdbsum/4ic5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ic5 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ic5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ic5 OCA], [http://pdbe.org/4ic5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ic5 RCSB], [http://www.ebi.ac.uk/pdbsum/4ic5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ic5 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/DEGP5_ARATH DEGP5_ARATH]] Probable serine protease. | + | [https://www.uniprot.org/uniprot/DEGP5_ARATH DEGP5_ARATH] Probable serine protease. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Arath]] | + | [[Category: Arabidopsis thaliana]] |
| - | [[Category: Fan, H]] | + | [[Category: Large Structures]] |
| - | [[Category: Gao, F]] | + | [[Category: Fan H]] |
| - | [[Category: Gong, W]] | + | [[Category: Gao F]] |
| - | [[Category: Liu, L]] | + | [[Category: Gong W]] |
| - | [[Category: Sun, W]] | + | [[Category: Liu L]] |
| - | [[Category: Bets-barrel]]
| + | [[Category: Sun W]] |
| - | [[Category: Calcium binding]]
| + | |
| - | [[Category: Chloroplast]]
| + | |
| - | [[Category: Endopeptidase]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| Structural highlights
Function
DEGP5_ARATH Probable serine protease.
Publication Abstract from PubMed
Plant Deg5 and Deg8 are two members of the HtrA proteases, a family of oligomeric serine endopeptidases that are involved in a variety of protein quality-control processes. These two HtrA proteases are located in the thylakoid lumen and participate in high-light stress responses by collaborating with other chloroplast proteins. Deg5 and Deg8 degrade photodamaged D1 protein of the photosystem II reaction centre, allowing its in situ replacement. Here, the crystal structures of Arabidopsis thaliana Deg5 (S266A) and Deg8 (S292A) are reported at 2.6 and 2.0 A resolution, respectively. The Deg5 trimer contains two calcium ions in a central channel, suggesting a link between photodamage control and calcium ions in chloroplasts. Previous structures of HtrA proteases have indicated that their regulation usually requires C-terminal PDZ domain(s). Deg5 is unique in that it contains no PDZ domain and the trimeric structure of Deg5 (S266A) reveals a novel catalytic triad conformation. A similar triad conformation is observed in the hexameric structure of the single PDZ-domain-containing Deg8 (S292A). These findings suggest a novel activation mechanism for plant HtrA proteases and provide structural clues to their function in light-stress response.
The structures of Arabidopsis Deg5 and Deg8 reveal new insights into HtrA proteases.,Sun W, Gao F, Fan H, Shan X, Sun R, Liu L, Gong W Acta Crystallogr D Biol Crystallogr. 2013 May;69(Pt 5):830-7. doi:, 10.1107/S0907444913002023. Epub 2013 Apr 11. PMID:23633592[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Sun W, Gao F, Fan H, Shan X, Sun R, Liu L, Gong W. The structures of Arabidopsis Deg5 and Deg8 reveal new insights into HtrA proteases. Acta Crystallogr D Biol Crystallogr. 2013 May;69(Pt 5):830-7. doi:, 10.1107/S0907444913002023. Epub 2013 Apr 11. PMID:23633592 doi:10.1107/S0907444913002023
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