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3wyg

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Revision as of 09:25, 21 February 2019

Crystal structure of Xpo1p-PKI-Gsp1p-GTP complex

Structural highlights

3wyg is a 3 chain structure with sequence from Baker's yeast and Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	3wyf
Gene:	AWRI796_3356 (Baker's yeast), CRM1, G8514, KAP124, XPO1, YGR218W (Baker's yeast), PKIA, PRKACN1 (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[IPKA_HUMAN] Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains. [XPO1_YEAST] Receptor for the leucine-rich nuclear export signal (NES).

Publication Abstract from PubMed

Proteins and ribonucleoproteins containing a nuclear export signal (NES) assemble with the exportin Xpo1p (yeast CRM1) and Gsp1p-GTP (yeast Ran-GTP) in the nucleus and exit through the nuclear pore complex. In the cytoplasm, Yrb1p (yeast RanBP1) displaces NES from Xpo1p. Efficient export of NES-cargoes requires Yrb2p (yeast RanBP3), a primarily nuclear protein containing nucleoporin-like phenylalanine-glycine (FG) repeats and a low-affinity Gsp1p-binding domain (RanBD). Here, we show that Yrb2p strikingly accelerates the association of Gsp1p-GTP and NES to Xpo1p. We have solved the crystal structure of the Xpo1p-Yrb2p-Gsp1p-GTP complex, a key assembly intermediate that can bind cargo rapidly. Although the NES-binding cleft of Xpo1p is closed in this intermediate, our data suggest that preloading of Gsp1p-GTP onto Xpo1p by Yrb2p, conformational flexibility of Xpo1p, and the low affinity of RanBD enable active displacement of Yrb2p RanBD by NES to occur effectively. The structure also reveals the major binding sites for FG repeats on Xpo1p.

Structural insights into how yrb2p accelerates the assembly of the xpo1p nuclear export complex.,Koyama M, Shirai N, Matsuura Y Cell Rep. 2014 Nov 6;9(3):983-95. doi: 10.1016/j.celrep.2014.09.052. Epub 2014, Oct 30. PMID:25437554^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Koyama M, Shirai N, Matsuura Y. Structural insights into how yrb2p accelerates the assembly of the xpo1p nuclear export complex. Cell Rep. 2014 Nov 6;9(3):983-95. doi: 10.1016/j.celrep.2014.09.052. Epub 2014, Oct 30. PMID:25437554 doi:http://dx.doi.org/10.1016/j.celrep.2014.09.052

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3wyg"

@@ Line 3: / Line 3: @@
 <StructureSection load='3wyg' size='340' side='right' caption='[[3wyg]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3wyg]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WYG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WYG FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3wyg]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WYG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WYG FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3wyf|3wyf]]</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AWRI796_3356 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=764097 Baker's yeast]), CRM1, G8514, KAP124, XPO1, YGR218W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), PKIA, PRKACN1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wyg OCA], [http://pdbe.org/3wyg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wyg RCSB], [http://www.ebi.ac.uk/pdbsum/3wyg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wyg ProSAT]</span></td></tr>
 </table>
@@ Line 21: / Line 22: @@
 ==See Also==
+*[[CAMP-dependent protein kinase|CAMP-dependent protein kinase]]
 *[[Exportin|Exportin]]
 == References ==
@@ Line 26: / Line 28: @@
 __TOC__
 </StructureSection>
+[[Category: Baker's yeast]]
+[[Category: Human]]
 [[Category: Koyama, M]]
 [[Category: Matsuura, Y]]

3wyg

From Proteopedia

Revision as of 09:25, 21 February 2019

Crystal structure of Xpo1p-PKI-Gsp1p-GTP complex

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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