4bh9

From Proteopedia

(Difference between revisions)

Revision as of 13:55, 13 March 2019

A structural model of CAP mutant (T127L and S128I) in the apo state

Structural highlights

4bh9 is a 1 chain structure with sequence from "bacillus_coli"_migula_1895 "bacillus coli" migula 1895. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	4bhp
Gene:	crp, cap, csm ("Bacillus coli" Migula 1895)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CRP_ECOLI] This protein complexes with cyclic AMP and binds to specific DNA sites near the promoter to regulate the transcription of several catabolite-sensitive operons. The protein induces a severe bend in the DNA. Acts as a negative regulator of its own synthesis as well as for adenylate cyclase (cyaA), which generates cAMP.^[1]

Publication Abstract from PubMed

The ability to inhibit binding or enzymatic activity is key to preventing aberrant behaviors of proteins. Allosteric inhibition is desirable as it offers several advantages over competitive inhibition, but the mechanisms of action remain poorly understood in most cases. Here we show that allosteric inhibition can be effected by destabilizing a low-populated conformational state that serves as an on-pathway intermediate for ligand binding, without altering the protein's ground-state structure. As standard structural approaches are typically concerned with changes in the ground-state structure of proteins, the presence of such a mechanism can go easily undetected. Our data strongly argue for the routine use of NMR tools suited to detect and characterize transiently formed conformational states in allosteric systems. Structure information on such important intermediates can ultimately result in more efficient design of allosteric inhibitors.

Allosteric inhibition through suppression of transient conformational states.,Tzeng SR, Kalodimos CG Nat Chem Biol. 2013 May 5. doi: 10.1038/nchembio.1250. PMID:23644478^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Aiba H. Transcription of the Escherichia coli adenylate cyclase gene is negatively regulated by cAMP-cAMP receptor protein. J Biol Chem. 1985 Mar 10;260(5):3063-70. PMID:2982847
↑ Tzeng SR, Kalodimos CG. Allosteric inhibition through suppression of transient conformational states. Nat Chem Biol. 2013 May 5. doi: 10.1038/nchembio.1250. PMID:23644478 doi:10.1038/nchembio.1250

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4bh9"

Categories: Bacillus coli migula 1895 | Large Structures | Kalodimos, C G | Tzeng, S R | Transcription

@@ Line 1: / Line 1: @@
 ==A structural model of CAP mutant (T127L and S128I) in the apo state==
-<StructureSection load='4bh9' size='340' side='right' caption='[[4bh9]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='4bh9' size='340' side='right'caption='[[4bh9]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4bh9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BH9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BH9 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4bh9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BH9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BH9 FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4bhp|4bhp]]</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">crp, cap, csm ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bh9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bh9 OCA], [http://pdbe.org/4bh9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bh9 RCSB], [http://www.ebi.ac.uk/pdbsum/4bh9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bh9 ProSAT]</span></td></tr>
 </table>
@@ Line 25: / Line 26: @@
 __TOC__
 </StructureSection>
-[[Category: Escherichia coli]]
+[[Category: Bacillus coli migula 1895]]
+[[Category: Large Structures]]
 [[Category: Kalodimos, C G]]
 [[Category: Tzeng, S R]]
 [[Category: Transcription]]

4bh9

From Proteopedia

Revision as of 13:55, 13 March 2019

A structural model of CAP mutant (T127L and S128I) in the apo state

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox