3nnm

From Proteopedia

(Difference between revisions)

Revision as of 00:32, 28 December 2023

Halogenase domain from CurA module (crystal form IV)

Structural highlights

3nnm is a 2 chain structure with sequence from Lyngbya majuscula. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.69Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q6DNF2_9CYAN

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The CurA halogenase (Hal) catalyzes a cryptic chlorination leading to cyclopropane ring formation in the synthesis of the natural product curacin A. Hal belongs to a family of enzymes that use Fe(2+), O(2) and alpha-ketoglutarate (alphaKG) to perform a variety of halogenation reactions in natural product biosynthesis. Crystal structures of the enzyme in five ligand states reveal strikingly different open and closed conformations dependent on alphaKG binding. The open form represents ligand-free enzyme, preventing substrate from entering the active site until both alphaKG and chloride are bound, while the closed form represents the holoenzyme with alphaKG and chloride coordinated to iron. Candidate amino acid residues involved in substrate recognition were identified by site-directed mutagenesis. These new structures provide direct evidence of a conformational switch driven by alphaKG leading to chlorination of an early pathway intermediate.

Conformational switch triggered by alpha-ketoglutarate in a halogenase of curacin A biosynthesis.,Khare D, Wang B, Gu L, Razelun J, Sherman DH, Gerwick WH, Hakansson K, Smith JL Proc Natl Acad Sci U S A. 2010 Aug 10;107(32):14099-104. Epub 2010 Jul 26. PMID:20660778^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Khare D, Wang B, Gu L, Razelun J, Sherman DH, Gerwick WH, Hakansson K, Smith JL. Conformational switch triggered by alpha-ketoglutarate in a halogenase of curacin A biosynthesis. Proc Natl Acad Sci U S A. 2010 Aug 10;107(32):14099-104. Epub 2010 Jul 26. PMID:20660778

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3nnm"

Categories: Large Structures | Lyngbya majuscula | Khare D | Smith JL

@@ Line 1: / Line 1: @@
 ==Halogenase domain from CurA module (crystal form IV)==
-<StructureSection load='3nnm' size='340' side='right' caption='[[3nnm]], [[Resolution|resolution]] 2.69&Aring;' scene=''>
+<StructureSection load='3nnm' size='340' side='right'caption='[[3nnm]], [[Resolution|resolution]] 2.69&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3nnm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lyngbya_majuscula Lyngbya majuscula]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NNM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NNM FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3nnm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lyngbya_majuscula Lyngbya majuscula]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NNM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NNM FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.69&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3nnf|3nnf]], [[3nnj|3nnj]], [[3nnl|3nnl]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">curA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158786 Lyngbya majuscula])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nnm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nnm OCA], [https://pdbe.org/3nnm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nnm RCSB], [https://www.ebi.ac.uk/pdbsum/3nnm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nnm ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nnm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nnm OCA], [http://pdbe.org/3nnm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3nnm RCSB], [http://www.ebi.ac.uk/pdbsum/3nnm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3nnm ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q6DNF2_9CYAN Q6DNF2_9CYAN]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nn/3nnm_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nn/3nnm_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 32: / Line 33: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Lyngbya majuscula]]
-[[Category: Khare, D]]
+[[Category: Khare D]]
-[[Category: Smith, J L]]
+[[Category: Smith JL]]
-[[Category: Biosynthetic protein]]
-[[Category: Catalyzes a cryptic chlorination]]

3nnm

From Proteopedia

Revision as of 00:32, 28 December 2023

Halogenase domain from CurA module (crystal form IV)

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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