1bb6
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1bb6.gif|left|200px]]<br /> | + | [[Image:1bb6.gif|left|200px]]<br /><applet load="1bb6" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1bb6" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1bb6, resolution 2.0Å" /> | caption="1bb6, resolution 2.0Å" /> | ||
'''LYSOZYME COMPLEX WITH 4-METHYL-UMBELLIFERYL CHITOTRIOSE'''<br /> | '''LYSOZYME COMPLEX WITH 4-METHYL-UMBELLIFERYL CHITOTRIOSE'''<br /> | ||
| Line 8: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1BB6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Oncorhynchus_mykiss Oncorhynchus mykiss] with UMG as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] | + | 1BB6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Oncorhynchus_mykiss Oncorhynchus mykiss] with UMG as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Known structural/functional Site: <scene name='pdbsite=NUL:Catalytically Active Residues'>NUL</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BB6 OCA]. |
==Reference== | ==Reference== | ||
| Line 23: | Line 22: | ||
[[Category: umbelliferone glycosides]] | [[Category: umbelliferone glycosides]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 14:25:35 2007'' |
Revision as of 12:15, 18 December 2007
|
LYSOZYME COMPLEX WITH 4-METHYL-UMBELLIFERYL CHITOTRIOSE
Overview
Two complexes between rainbow trout lysozyme (RBTL) and, 4-methylumbelliferyl chitobioside, 4MeU-(GlcNAc)2, and chitotrioside, 4MeU-(GlcNAc)3, were produced by co-crystallization and soaking, respectively, and the crystal structures were solved at 2.0 A resolution., The results show that 4-MeU-(GlcNAc)3 binds in subsites A-D and that, 4-MeU-(GlcNAc)2 binds in subsites B-D in the active-site cleft of RBTL., This agrees well with earlier crystallographic studies on the binding of, oligosaccharides of chitin to RBTL, which showed that (GlcNAc)3 binds to, sites B-D in RBTL and not to A-C as seen in the human and turkey egg-white, lysozymes. For both complexes the 4-MeU moiety in site D has diffuse, electron density and is flexible, as it is only bound to water molecules, and not to the protein. Since no electron density was observed in site E, the solved structures give views of nonproductive enzyme-substrate, complexes.
About this Structure
1BB6 is a Single protein structure of sequence from Oncorhynchus mykiss with UMG as ligand. Active as Lysozyme, with EC number 3.2.1.17 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structural studies on the binding of 4-methylumbelliferone glycosides of chitin to rainbow trout lysozyme., Vollan VB, Hough E, Karlsen S, Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):60-6. Epub 1999 Jan, 1. PMID:10089395
Page seeded by OCA on Tue Dec 18 14:25:35 2007
