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| | ==Crystal Structure of Yeast Irc6p - A Novel Type of Conserved Clathrin Accessory Protein== | | ==Crystal Structure of Yeast Irc6p - A Novel Type of Conserved Clathrin Accessory Protein== |
| - | <StructureSection load='3uc9' size='340' side='right' caption='[[3uc9]], [[Resolution|resolution]] 1.80Å' scene=''> | + | <StructureSection load='3uc9' size='340' side='right'caption='[[3uc9]], [[Resolution|resolution]] 1.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3uc9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UC9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UC9 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3uc9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UC9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UC9 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">IRC6, YFR043C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">IRC6, YFR043C ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uc9 OCA], [http://pdbe.org/3uc9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3uc9 RCSB], [http://www.ebi.ac.uk/pdbsum/3uc9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3uc9 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uc9 OCA], [https://pdbe.org/3uc9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uc9 RCSB], [https://www.ebi.ac.uk/pdbsum/3uc9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uc9 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/IRC6_YEAST IRC6_YEAST]] Involved in gross chromosomal rearrangements (GCRs) and telomere healing.<ref>PMID:20194442</ref> | + | [[https://www.uniprot.org/uniprot/IRC6_YEAST IRC6_YEAST]] Involved in gross chromosomal rearrangements (GCRs) and telomere healing.<ref>PMID:20194442</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Baker's yeast]] | | [[Category: Baker's yeast]] |
| | + | [[Category: Large Structures]] |
| | [[Category: Cascio, D]] | | [[Category: Cascio, D]] |
| | [[Category: Gorynia, S]] | | [[Category: Gorynia, S]] |
| Structural highlights
Function
[IRC6_YEAST] Involved in gross chromosomal rearrangements (GCRs) and telomere healing.[1]
Publication Abstract from PubMed
Clathrin coat accessory proteins play key roles in transport mediated by clathrin-coated vesicles. Yeast Irc6p and the related mammalian p34 are putative clathrin accessory proteins that interact with clathrin adaptor complexes. Here we present evidence that Irc6p functions in clathrin-mediated traffic between the TGN and endosomes, linking clathrin adaptor complex AP-1 and the Rab GTPase Ypt31p. The crystal structure of the Irc6p N-terminal domain revealed a G protein fold most related to small G proteins of the Rab and Arf families. However, Irc6p lacks G protein signature motifs and high-affinity GTP binding. Also, mutant Irc6p lacking candidate GTP-binding residues retained function. Mammalian p34 rescued growth defects in irc6 cells indicating functional conservation, and modeling predicted a similar N-terminal fold in p34. Irc6p and p34 also contain functionally conserved C-terminal regions. Irc6p/p34-related proteins with the same two-part architecture are encoded in genomes of species as diverse as plants and humans. Together these results define Irc6p/p34 as a novel type of conserved clathrin accessory protein and founding members of a new G protein-like family.
Yeast Irc6p is a novel type of conserved clathrin coat accessory factor related to small G proteins.,Gorynia S, Lorenz TC, Costaguta G, Daboussi L, Cascio D, Payne GS Mol Biol Cell. 2012 Sep 19. PMID:22993212[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Zhang W, Durocher D. De novo telomere formation is suppressed by the Mec1-dependent inhibition of Cdc13 accumulation at DNA breaks. Genes Dev. 2010 Mar 1;24(5):502-15. doi: 10.1101/gad.1869110. PMID:20194442 doi:http://dx.doi.org/10.1101/gad.1869110
- ↑ Gorynia S, Lorenz TC, Costaguta G, Daboussi L, Cascio D, Payne GS. Yeast Irc6p is a novel type of conserved clathrin coat accessory factor related to small G proteins. Mol Biol Cell. 2012 Sep 19. PMID:22993212 doi:http://dx.doi.org/10.1091/mbc.E12-07-0507
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