3hb6

Publication Abstract from PubMed

BACKGROUND: Heme oxidative degradation has been extensively investigated in peroxidases but not in catalases. The verdoheme formation, a product of heme oxidation which inactivates the enzyme, was studied in Proteus mirabilis catalase. METHODS: The verdoheme was generated by adding peracetic acid and analyzed by mass spectrometry and spectrophotometry. RESULTS: Kinetics follow-up of different catalase reactional intermediates shows that i) the formation of compound I always precedes that of verdoheme, ii) compound III is never observed, iii) the rate of compound II decomposition is not compatible with that of verdoheme formation, and iv) dithiothreitol prevents the verdoheme formation but not that of compound II, whereas NADPH prevents both of them. The formation of verdoheme is strongly inhibited by EDTA but not increased by Fe3+ or Cu2+ salts. The generation of verdoheme is facilitated by the presence of protein radicals as observed in the F194Y mutated catalase. The inability of the inactive variant (H54F) to form verdoheme, indicates that the heme oxidation is fully associated to the enzyme catalysis. CONCLUSION: These data, taken together, strongly suggest that the verdoheme formation pathway originates from compound I rather than from compound II. GENERAL SIGNIFICANCE: The autocatalytic verdoheme formation is likely to occur in vivo.

Verdoheme formation in Proteus mirabilis catalase.,Andreoletti P, Mouesca JM, Gouet P, Jaquinod M, Capeillere-Blandin C, Jouve HM Biochim Biophys Acta. 2009 Aug;1790(8):741-53. Epub 2009 Apr 24. PMID:19394409^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.