1mj1
From Proteopedia
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[[Image:1mj1.gif|left|200px]] | [[Image:1mj1.gif|left|200px]] | ||
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'''FITTING THE TERNARY COMPLEX OF EF-Tu/tRNA/GTP AND RIBOSOMAL PROTEINS INTO A 13 A CRYO-EM MAP OF THE COLI 70S RIBOSOME''' | '''FITTING THE TERNARY COMPLEX OF EF-Tu/tRNA/GTP AND RIBOSOMAL PROTEINS INTO A 13 A CRYO-EM MAP OF THE COLI 70S RIBOSOME''' | ||
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[[Category: Zemlin, F.]] | [[Category: Zemlin, F.]] | ||
[[Category: 70s ribosome]] | [[Category: 70s ribosome]] | ||
| - | [[Category: | + | [[Category: Ef-tu]] |
| - | [[Category: | + | [[Category: Low resolution model ternary complex]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:08:52 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 22:08, 2 May 2008
FITTING THE TERNARY COMPLEX OF EF-Tu/tRNA/GTP AND RIBOSOMAL PROTEINS INTO A 13 A CRYO-EM MAP OF THE COLI 70S RIBOSOME
Overview
The mRNA codon in the ribosomal A-site is recognized by aminoacyl-tRNA (aa-tRNA) in a ternary complex with elongation factor Tu (EF-Tu) and GTP. Here we report the 13 A resolution three-dimensional reconstruction determined by cryo-electron microscopy of the kirromycin-stalled codon-recognition complex. The structure of the ternary complex is distorted by binding of the tRNA anticodon arm in the decoding center. The aa-tRNA interacts with 16S rRNA, helix 69 of 23S rRNA and proteins S12 and L11, while the sarcin-ricin loop of 23S rRNA contacts domain 1 of EF-Tu near the nucleotide-binding pocket. These results provide a detailed snapshot view of an important functional state of the ribosome and suggest mechanisms of decoding and GTPase activation.
About this Structure
1MJ1 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Ribosome interactions of aminoacyl-tRNA and elongation factor Tu in the codon-recognition complex., Stark H, Rodnina MV, Wieden HJ, Zemlin F, Wintermeyer W, van Heel M, Nat Struct Biol. 2002 Nov;9(11):849-54. PMID:12379845 Page seeded by OCA on Sat May 3 01:08:52 2008
