1mk0
From Proteopedia
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'''catalytic domain of intron endonuclease I-TevI, E75A mutant''' | '''catalytic domain of intron endonuclease I-TevI, E75A mutant''' | ||
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[[Category: Meehan, L.]] | [[Category: Meehan, L.]] | ||
[[Category: Roey, P Van.]] | [[Category: Roey, P Van.]] | ||
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| - | [[Category: | + | [[Category: Catalytic domain]] |
| - | [[Category: | + | [[Category: Dna-binding surface]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:15:00 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 22:15, 2 May 2008
catalytic domain of intron endonuclease I-TevI, E75A mutant
Overview
I-TevI, a member of the GIY-YIG family of homing endonucleases, consists of an N-terminal catalytic domain and a C-terminal DNA-binding domain joined by a flexible linker. The GIY-YIG motif is in the N-terminal domain of I-TevI, which corresponds to a phylogenetically widespread catalytic cartridge that is often associated with mobile genetic elements. The crystal structure of the catalytic domain of I-TevI, the first of any GIY-YIG endonuclease, reveals a novel alpha/beta-fold with a central three-stranded antiparallel beta-sheet flanked by three helices. The most conserved and putative catalytic residues are located on a shallow, concave surface and include a metal coordination site. Similarities in the three-dimensional arrangement of the catalytically important residues and the cation-binding site with those of the His-Cys box endonuclease I-PpoI suggest the possibility of mechanistic relationships among these different families of homing endonucleases despite completely different folds.
About this Structure
1MK0 is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.
Reference
Catalytic domain structure and hypothesis for function of GIY-YIG intron endonuclease I-TevI., Van Roey P, Meehan L, Kowalski JC, Belfort M, Derbyshire V, Nat Struct Biol. 2002 Nov;9(11):806-11. PMID:12379841 Page seeded by OCA on Sat May 3 01:15:00 2008
