1mmc
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1mmc.gif|left|200px]] | [[Image:1mmc.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1mmc", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_1mmc| PDB=1mmc | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''1H NMR STUDY OF THE SOLUTION STRUCTURE OF AC-AMP2''' | '''1H NMR STUDY OF THE SOLUTION STRUCTURE OF AC-AMP2''' | ||
| Line 32: | Line 29: | ||
[[Category: Willem, R.]] | [[Category: Willem, R.]] | ||
[[Category: Wyns, L.]] | [[Category: Wyns, L.]] | ||
| - | [[Category: | + | [[Category: Antifungal antimicrobial]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:24:57 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 22:24, 2 May 2008
1H NMR STUDY OF THE SOLUTION STRUCTURE OF AC-AMP2
Overview
The conformation in water of antimicrobial protein 2 from Amaranthus caudatus (Ac-AMP2) was determined using 1H NMR, DIANA and restrained molecular modeling. Ac-AMP2 is a 30 amino acid residue, lectin-like protein that specifically binds to chitin, a polymer of beta-1,4-N-acetyl-D-glucosamine. After sequence specific resonance assignments, a total of 198 distance restraints were collected from 2D NOESY buildup spectra at 500 MHz at pH 2, supplemented by a 2D NOESY spectrum at 600 MHz. The location of the three previously unassigned disulfide bridges was determined from preliminary DIANA structures, using a statistical analysis of intercystinyl distances. The solution structure of Ac-AMP2 is presented as a set of 26 DIANA structures, further refined by restrained molecular dynamics using a simulated annealing protocol in the AMBER force field, with a backbone r.m.s.d. for the well defined Glu3-Cys28 segment of 0.69(+/-0.12) angstroms. The main structural element is an antiparallel beta-sheet from Met13 to Lys23 including a betaI-turn over Gln17-Phel8 with a beta bulge at Gly19. In addition, a beta'I turn over Arg6-Gly7, a beta'III turn over Ser11-Gly12 and a helical turn from Gly24 to Cys28 are identified. This structure is very similar to the equivalent regions of the X-ray structure of wheat germ agglutinin and the NMR structure of hevein.
About this Structure
1MMC is a Single protein structure of sequence from Amaranthus caudatus. Full crystallographic information is available from OCA.
Reference
H NMR study of the solution structure of Ac-AMP2, a sugar binding antimicrobial protein isolated from Amaranthus caudatus., Martins JC, Maes D, Loris R, Pepermans HA, Wyns L, Willem R, Verheyden P, J Mol Biol. 1996 May 3;258(2):322-33. PMID:8627629 Page seeded by OCA on Sat May 3 01:24:57 2008
