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| | ==Major mouse urinary protein IV complexed with 2,5-dimethylpyrazine== | | ==Major mouse urinary protein IV complexed with 2,5-dimethylpyrazine== |
| - | <StructureSection load='3kfi' size='340' side='right' caption='[[3kfi]], [[Resolution|resolution]] 1.42Å' scene=''> | + | <StructureSection load='3kfi' size='340' side='right'caption='[[3kfi]], [[Resolution|resolution]] 1.42Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3kfi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KFI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KFI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3kfi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KFI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KFI FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=25R:2,5-DIMETHYLPYRAZINE'>25R</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.42Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3kff|3kff]], [[3kfg|3kfg]], [[3kfh|3kfh]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=25R:2,5-DIMETHYLPYRAZINE'>25R</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Mup4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3kfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kfi OCA], [https://pdbe.org/3kfi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3kfi RCSB], [https://www.ebi.ac.uk/pdbsum/3kfi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3kfi ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kfi OCA], [http://pdbe.org/3kfi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3kfi RCSB], [http://www.ebi.ac.uk/pdbsum/3kfi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3kfi ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MUP4_MOUSE MUP4_MOUSE]] Binds pheromones, likely to displace pheromones complexed to urinary MUPs and transport them to the vomeronasal organ (VNO) where they associate with their neuronal receptor(s). MUP4 is highly specific for the male mouse pheromone 2-sec-butyl-4,5-dihydrothiazole (SBT).<ref>PMID:12192080</ref> <ref>PMID:20509168</ref> | + | [https://www.uniprot.org/uniprot/MUP4_MOUSE MUP4_MOUSE] Binds pheromones, likely to displace pheromones complexed to urinary MUPs and transport them to the vomeronasal organ (VNO) where they associate with their neuronal receptor(s). MUP4 is highly specific for the male mouse pheromone 2-sec-butyl-4,5-dihydrothiazole (SBT).<ref>PMID:12192080</ref> <ref>PMID:20509168</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
| | Check<jmol> | | Check<jmol> |
| | <jmolCheckbox> | | <jmolCheckbox> |
| - | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kf/3kfi_consurf.spt"</scriptWhenChecked> | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kf/3kfi_consurf.spt"</scriptWhenChecked> |
| | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> | | <text>to colour the structure by Evolutionary Conservation</text> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Large Structures]] |
| - | [[Category: Hurley, T D]] | + | [[Category: Mus musculus]] |
| - | [[Category: Perez-Miller, S]] | + | [[Category: Hurley TD]] |
| - | [[Category: Zou, Q]] | + | [[Category: Perez-Miller S]] |
| - | [[Category: Beta barrel]]
| + | [[Category: Zou Q]] |
| - | [[Category: Disulfide bond]]
| + | |
| - | [[Category: Lipocalin]]
| + | |
| - | [[Category: Major urinary protein]]
| + | |
| - | [[Category: Pheromone]]
| + | |
| - | [[Category: Pheromone-binding]]
| + | |
| - | [[Category: Secreted]] | + | |
| - | [[Category: Transport]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| Structural highlights
Function
MUP4_MOUSE Binds pheromones, likely to displace pheromones complexed to urinary MUPs and transport them to the vomeronasal organ (VNO) where they associate with their neuronal receptor(s). MUP4 is highly specific for the male mouse pheromone 2-sec-butyl-4,5-dihydrothiazole (SBT).[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
In mice, the major urinary proteins (MUP) play a key role in pheromonal communication by binding and transporting semiochemicals. MUP-IV is the only isoform known to be expressed in the vomeronasal mucosa. In comparison to the MUP isoforms that are abundantly excreted in the urine, MUP-IV is highly specific for the male mouse pheromone 2-sec-butyl-4,5-dihydrothiazole (SBT). In order to examine the structural basis of this ligand preference, we determined the X-ray crystal structure of MUP-IV bound to three mouse pheromones: SBT, 2,5-dimethylpyrazine (DMP), and 2-heptanone (HT). We also obtained the structure of MUP-IV with 2-ethylhexanol bound in the cavity. These four structures show that, relative to the major excreted MUP isoforms, three amino acid substitutions within the binding calyx impact ligand coordination. The F103 for A along with F54 for L result in a smaller cavity, potentially creating a more closely packed environment for the ligand. The E118 for G substitution introduces a charged group into a hydrophobic environment. The sidechain of E118 is observed to hydrogen-bond to polar groups on all four ligands with nearly the same geometry as seen for the water-mediated hydrogen bond network in the MUP-I and MUP-II crystal structures. These differences in cavity size and interactions between the protein and ligand are likely to contribute to the observed specificity of MUP-IV.
High resolution X-ray structures of mouse major urinary protein nasal isoform in complex with pheromones.,Perez-Miller S, Zou Q, Novotny MV, Hurley TD Protein Sci. 2010 May 27. PMID:20509168[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Sharrow SD, Vaughn JL, Zidek L, Novotny MV, Stone MJ. Pheromone binding by polymorphic mouse major urinary proteins. Protein Sci. 2002 Sep;11(9):2247-56. PMID:12192080 doi:http://dx.doi.org/10.1110/ps.0204202
- ↑ Perez-Miller S, Zou Q, Novotny MV, Hurley TD. High resolution X-ray structures of mouse major urinary protein nasal isoform in complex with pheromones. Protein Sci. 2010 May 27. PMID:20509168 doi:10.1002/pro.426
- ↑ Perez-Miller S, Zou Q, Novotny MV, Hurley TD. High resolution X-ray structures of mouse major urinary protein nasal isoform in complex with pheromones. Protein Sci. 2010 May 27. PMID:20509168 doi:10.1002/pro.426
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