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| | ==Structure of a stabilised ceSAS-6 dimer== | | ==Structure of a stabilised ceSAS-6 dimer== |
| - | <StructureSection load='4geu' size='340' side='right' caption='[[4geu]], [[Resolution|resolution]] 2.65Å' scene=''> | + | <StructureSection load='4geu' size='340' side='right'caption='[[4geu]], [[Resolution|resolution]] 2.65Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4geu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Caeel Caeel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GEU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GEU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4geu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GEU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GEU FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4g79|4g79]], [[2y3v|2y3v]], [[2y3w|2y3w]], [[3pyi|3pyi]], [[3q0y|3q0y]], [[3q0x|3q0x]], [[4gex|4gex]], [[4gfa|4gfa]], [[4gfc|4gfc]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4geu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4geu OCA], [https://pdbe.org/4geu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4geu RCSB], [https://www.ebi.ac.uk/pdbsum/4geu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4geu ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sas-6, Y45F10D.9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4geu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4geu OCA], [http://pdbe.org/4geu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4geu RCSB], [http://www.ebi.ac.uk/pdbsum/4geu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4geu ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SAS6_CAEEL SAS6_CAEEL]] Required for centrosome duplication. Participates in centriole assembly.<ref>PMID:15572125</ref> <ref>PMID:15665853</ref> | + | [https://www.uniprot.org/uniprot/SAS6_CAEEL SAS6_CAEEL] Required for centrosome duplication. Participates in centriole assembly.<ref>PMID:15572125</ref> <ref>PMID:15665853</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Caeel]] | + | [[Category: Caenorhabditis elegans]] |
| - | [[Category: Erat, M C]] | + | [[Category: Large Structures]] |
| - | [[Category: Vakonakis, I]] | + | [[Category: Erat MC]] |
| - | [[Category: Alpha-beta protein]] | + | [[Category: Vakonakis I]] |
| - | [[Category: Beta-sandwich]]
| + | |
| - | [[Category: Centriolar]]
| + | |
| - | [[Category: Cytoplasmic]]
| + | |
| - | [[Category: Structural protein]]
| + | |
| Structural highlights
Function
SAS6_CAEEL Required for centrosome duplication. Participates in centriole assembly.[1] [2]
Publication Abstract from PubMed
Centrioles are evolutionary conserved organelles that give rise to cilia and flagella as well as centrosomes. Centrioles display a characteristic ninefold symmetry imposed by the spindle assembly abnormal protein 6 (SAS-6) family. SAS-6 from Chlamydomonas reinhardtii and Danio rerio was shown to form ninefold symmetric, ring-shaped oligomers in vitro that were similar to the cartwheels observed in vivo during early steps of centriole assembly in most species. Here, we report crystallographic and EM analyses showing that, instead, Caenorhabotis elegans SAS-6 self-assembles into a spiral arrangement. Remarkably, we find that this spiral arrangement is also consistent with ninefold symmetry, suggesting that two distinct SAS-6 oligomerization architectures can direct the same output symmetry. Sequence analysis suggests that SAS-6 spirals are restricted to specific nematodes. This oligomeric arrangement may provide a structural basis for the presence of a central tube instead of a cartwheel during centriole assembly in these species.
Caenorhabditis elegans centriolar protein SAS-6 forms a spiral that is consistent with imparting a ninefold symmetry.,Hilbert M, Erat MC, Hachet V, Guichard P, Blank ID, Fluckiger I, Slater L, Lowe ED, Hatzopoulos GN, Steinmetz MO, Gonczy P, Vakonakis I Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11373-8. doi:, 10.1073/pnas.1302721110. Epub 2013 Jun 24. PMID:23798409[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Dammermann A, Muller-Reichert T, Pelletier L, Habermann B, Desai A, Oegema K. Centriole assembly requires both centriolar and pericentriolar material proteins. Dev Cell. 2004 Dec;7(6):815-29. PMID:15572125 doi:S1534580704003806
- ↑ Leidel S, Delattre M, Cerutti L, Baumer K, Gonczy P. SAS-6 defines a protein family required for centrosome duplication in C. elegans and in human cells. Nat Cell Biol. 2005 Feb;7(2):115-25. PMID:15665853 doi:ncb1220
- ↑ Hilbert M, Erat MC, Hachet V, Guichard P, Blank ID, Fluckiger I, Slater L, Lowe ED, Hatzopoulos GN, Steinmetz MO, Gonczy P, Vakonakis I. Caenorhabditis elegans centriolar protein SAS-6 forms a spiral that is consistent with imparting a ninefold symmetry. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11373-8. doi:, 10.1073/pnas.1302721110. Epub 2013 Jun 24. PMID:23798409 doi:10.1073/pnas.1302721110
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