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4qcj

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Revision as of 09:40, 24 April 2019

Crystal Structure of OdhI from Corynebacterium glutamicum

Structural highlights

4qcj is a 1 chain structure with sequence from Corgl. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	cg1630, Cgl1441, odhI (CORGL)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ODHI_CORGL] An essential component of the PknG signaling pathway. When unphosphorylated, it inhibits the activity of 2-oxoglutarate dehydrogenase. When phosphorylated it does not inhibit 2-oxoglutarate dehydrogenase.

Publication Abstract from PubMed

Pyruvate dehydrogenase and oxoglutarate dehydrogenase catalyze key reactions in central metabolism. In Corynebacterium glutamicum and related bacteria like Mycobacterium tuberculosis both activities reside in a novel protein supercomplex with the fusion protein OdhA catalyzing the conversion of oxoglutarate to succinyl-coenzyme A. This activity is inhibited by the forkhead-associated (FHA) domain of the small autoinhibitory protein OdhI. Here we used a biological screen which enabled us to isolate suppressor mutants that are influenced in OdhA-OdhI interaction. Five mutants carrying an OdhI mutation were isolated and one with an OdhA mutation. The OdhA mutein OdhA-C704E and three additional C704 variants were constructed. They exhibited unaltered or even slightly enhanced OdhA activity but showed reduced inhibition and interaction with OdhI. The FHA domain of OdhI was crystallized and its structure found in full agreement with previously determined NMR structures. Based on further structural studies, OdhA-OdhI crosslinking experiments, and modeling we discuss the experimental data generated on OdhA-OdhI interaction, with the latter protein representing a rare example of an FHA domain also recognizing a non-phosphorylated interaction partner.

Interaction of 2-oxoglutarate dehydrogenase OdhA with its inhibitor OdhI in Corynebacterium glutamicum: Mutants and a model.,Raasch K, Bocola M, Labahn J, Leitner A, Eggeling L, Bott M J Biotechnol. 2014 Jun 4. pii: S0168-1656(14)00269-7. doi:, 10.1016/j.jbiotec.2014.05.023. PMID:24905147^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Raasch K, Bocola M, Labahn J, Leitner A, Eggeling L, Bott M. Interaction of 2-oxoglutarate dehydrogenase OdhA with its inhibitor OdhI in Corynebacterium glutamicum: Mutants and a model. J Biotechnol. 2014 Jun 4. pii: S0168-1656(14)00269-7. doi:, 10.1016/j.jbiotec.2014.05.023. PMID:24905147 doi:http://dx.doi.org/10.1016/j.jbiotec.2014.05.023

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4qcj"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of OdhI from Corynebacterium glutamicum==
-<StructureSection load='4qcj' size='340' side='right' caption='[[4qcj]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
+<StructureSection load='4qcj' size='340' side='right'caption='[[4qcj]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4qcj]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QCJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QCJ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4qcj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Corgl Corgl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QCJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QCJ FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qcj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qcj OCA], [http://pdbe.org/4qcj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qcj RCSB], [http://www.ebi.ac.uk/pdbsum/4qcj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qcj ProSAT]</span></td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cg1630, Cgl1441, odhI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=196627 CORGL])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qcj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qcj OCA], [http://pdbe.org/4qcj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qcj RCSB], [http://www.ebi.ac.uk/pdbsum/4qcj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qcj ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Corgl]]
+[[Category: Large Structures]]
 [[Category: Bocola, M]]
 [[Category: Bott, M]]

4qcj

From Proteopedia

Revision as of 09:40, 24 April 2019

Crystal Structure of OdhI from Corynebacterium glutamicum

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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