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3nfq

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Revision as of 07:01, 12 May 2022

Crystal structure of the conserved central domain of yeast Spn1/Iws1

Structural highlights

3nfq is a 2 chain structure with sequence from Atcc 18824. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:
Gene:	IWS1, SPN1, YPR133C (ATCC 18824)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[IWS1_YEAST] Transcription factor involved in RNA polymerase II transcription regulation. May function in both SPT15/TBP post-recruitment and recruitment steps of transcription.^[1] ^[2]

Publication Abstract from PubMed

Spn1 plays essential roles in the regulation of gene expression by RNA polymerase II (RNAPII), and it is highly conserved in organisms ranging from yeast to humans. Spn1 physically and/or genetically interacts with RNAPII, TBP (TATA-binding protein), TFIIS (transcription factor IIS), and a number of chromatin remodeling factors (Swi-Snf and Spt6). The central domain of Spn1 (residues 141-305 out of 410) is necessary and sufficient for performing the essential functions of SPN1 in yeast cells. Here, we report the high-resolution (1.85 A) crystal structure of the conserved central domain of Saccharomyces cerevisiae Spn1. The central domain is composed of eight alpha-helices in a right-handed superhelical arrangement and exhibits structural similarity to domain I of TFIIS. A unique structural feature of Spn1 is a highly conserved loop, which defines one side of a pronounced cavity. The loop and the other residues forming the cavity are highly conserved at the amino acid level among all Spn1 family members, suggesting that this is a signature motif for Spn1 orthologs. The locations and the molecular characterization of temperature-sensitive mutations in Spn1 indicate that the cavity is a key attribute of Spn1 that is critical for its regulatory functions during RNAPII-mediated transcriptional activity.

The Transcription Factor Spn1 Regulates Gene Expression via a Highly Conserved Novel Structural Motif.,Pujari V, Radebaugh CA, Chodaparambil JV, Muthurajan UM, Almeida AR, Fischbeck JA, Luger K, Stargell LA J Mol Biol. 2010 Sep 25. PMID:20875428^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Fischbeck JA, Kraemer SM, Stargell LA. SPN1, a conserved gene identified by suppression of a postrecruitment-defective yeast TATA-binding protein mutant. Genetics. 2002 Dec;162(4):1605-16. PMID:12524336
↑ Lindstrom DL, Squazzo SL, Muster N, Burckin TA, Wachter KC, Emigh CA, McCleery JA, Yates JR 3rd, Hartzog GA. Dual roles for Spt5 in pre-mRNA processing and transcription elongation revealed by identification of Spt5-associated proteins. Mol Cell Biol. 2003 Feb;23(4):1368-78. PMID:12556496
↑ Pujari V, Radebaugh CA, Chodaparambil JV, Muthurajan UM, Almeida AR, Fischbeck JA, Luger K, Stargell LA. The Transcription Factor Spn1 Regulates Gene Expression via a Highly Conserved Novel Structural Motif. J Mol Biol. 2010 Sep 25. PMID:20875428 doi:10.1016/j.jmb.2010.09.040

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3nfq"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the conserved central domain of yeast Spn1/Iws1==
-<StructureSection load='3nfq' size='340' side='right' caption='[[3nfq]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
+<StructureSection load='3nfq' size='340' side='right'caption='[[3nfq]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3nfq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NFQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NFQ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3nfq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NFQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NFQ FirstGlance]. <br>
 </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">IWS1, SPN1, YPR133C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">IWS1, SPN1, YPR133C ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nfq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nfq OCA], [http://pdbe.org/3nfq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3nfq RCSB], [http://www.ebi.ac.uk/pdbsum/3nfq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3nfq ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nfq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nfq OCA], [https://pdbe.org/3nfq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nfq RCSB], [https://www.ebi.ac.uk/pdbsum/3nfq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nfq ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/IWS1_YEAST IWS1_YEAST]] Transcription factor involved in RNA polymerase II transcription regulation. May function in both SPT15/TBP post-recruitment and recruitment steps of transcription.<ref>PMID:12524336</ref> <ref>PMID:12556496</ref>
+[[https://www.uniprot.org/uniprot/IWS1_YEAST IWS1_YEAST]] Transcription factor involved in RNA polymerase II transcription regulation. May function in both SPT15/TBP post-recruitment and recruitment steps of transcription.<ref>PMID:12524336</ref> <ref>PMID:12556496</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 24: / Line 24: @@
 </StructureSection>
 [[Category: Atcc 18824]]
+[[Category: Large Structures]]
 [[Category: Luger, K]]
 [[Category: Stargell, L A]]

3nfq

From Proteopedia

Revision as of 07:01, 12 May 2022

Crystal structure of the conserved central domain of yeast Spn1/Iws1

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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