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1muh

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[[Image:1muh.gif|left|200px]]
[[Image:1muh.gif|left|200px]]
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{{Structure
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The line below this paragraph, containing "STRUCTURE_1muh", creates the "Structure Box" on the page.
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>
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{{STRUCTURE_1muh| PDB=1muh | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1muh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1muh OCA], [http://www.ebi.ac.uk/pdbsum/1muh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1muh RCSB]</span>
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'''CRYSTAL STRUCTURE OF TN5 TRANSPOSASE COMPLEXED WITH TRANSPOSON END DNA'''
'''CRYSTAL STRUCTURE OF TN5 TRANSPOSASE COMPLEXED WITH TRANSPOSON END DNA'''
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==About this Structure==
==About this Structure==
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1MUH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry 1F3I. The following page contains interesting information on the relation of 1MUH with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb84_1.html Transposase]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MUH OCA].
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1MUH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1f3i 1f3i]. The following page contains interesting information on the relation of 1MUH with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb84_1.html Transposase]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MUH OCA].
==Reference==
==Reference==
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[[Category: Reznikoff, W S.]]
[[Category: Reznikoff, W S.]]
[[Category: Thoden, J B.]]
[[Category: Thoden, J B.]]
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[[Category: protein-dna complex]]
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[[Category: Protein-dna complex]]
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[[Category: ribonuclease h-like motif]]
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[[Category: Ribonuclease h-like motif]]
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[[Category: synaptic complex]]
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[[Category: Synaptic complex]]
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[[Category: transposase]]
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[[Category: Transposase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:43:59 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:21:15 2008''
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Revision as of 22:43, 2 May 2008

Image:1muh.gif

Template:STRUCTURE 1muh

CRYSTAL STRUCTURE OF TN5 TRANSPOSASE COMPLEXED WITH TRANSPOSON END DNA


Overview

Genomic evolution has been profoundly influenced by DNA transposition, a process whereby defined DNA segments move freely about the genome. Transposition is mediated by transposases, and similar events are catalyzed by retroviral integrases such as human immunodeficiency virus-1 (HIV-1) integrase. Understanding how these proteins interact with DNA is central to understanding the molecular basis of transposition. We report the three-dimensional structure of prokaryotic Tn5 transposase complexed with Tn5 transposon end DNA determined to 2.3 angstrom resolution. The molecular assembly is dimeric, where each double-stranded DNA molecule is bound by both protein subunits, orienting the transposon ends into the active sites. This structure provides a molecular framework for understanding many aspects of transposition, including the binding of transposon end DNA by one subunit and cleavage by a second, cleavage of two strands of DNA by a single active site via a hairpin intermediate, and strand transfer into target DNA.

About this Structure

1MUH is a Single protein structure of sequence from Escherichia coli. This structure supersedes the now removed PDB entry 1f3i. The following page contains interesting information on the relation of 1MUH with [Transposase]. Full crystallographic information is available from OCA.

Reference

Three-dimensional structure of the Tn5 synaptic complex transposition intermediate., Davies DR, Goryshin IY, Reznikoff WS, Rayment I, Science. 2000 Jul 7;289(5476):77-85. PMID:10884228 Page seeded by OCA on Sat May 3 01:43:59 2008

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