|
|
| Line 1: |
Line 1: |
| | | | |
| | ==Human actin-related protein Arp8 in its ATP-bound state== | | ==Human actin-related protein Arp8 in its ATP-bound state== |
| - | <StructureSection load='4fo0' size='340' side='right' caption='[[4fo0]], [[Resolution|resolution]] 2.60Å' scene=''> | + | <StructureSection load='4fo0' size='340' side='right'caption='[[4fo0]], [[Resolution|resolution]] 2.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4fo0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FO0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FO0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4fo0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FO0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FO0 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3qb0|3qb0]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fo0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fo0 OCA], [https://pdbe.org/4fo0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fo0 RCSB], [https://www.ebi.ac.uk/pdbsum/4fo0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fo0 ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ACTR8, ACTR8: ARP8 actin-related protein 8 homolog (yeast), ARP8, INO80N ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fo0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fo0 OCA], [http://pdbe.org/4fo0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4fo0 RCSB], [http://www.ebi.ac.uk/pdbsum/4fo0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4fo0 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ARP8_HUMAN ARP8_HUMAN]] Plays an important role in the functional organization of mitotic chromosomes. Exhibits low basal ATPase activity, and unable to polymerize. Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA reoplication and probably DNA repair. Required for the recruitment of INO80 (and probably the INO80 complex) to sites of DNA damage. Strongly prefer nucleosomes and H3-H4 tetramers over H2A-H2B dimers, suggesting it may act as a nucleosome recognition module within the complex. | + | [https://www.uniprot.org/uniprot/ARP8_HUMAN ARP8_HUMAN] Plays an important role in the functional organization of mitotic chromosomes. Exhibits low basal ATPase activity, and unable to polymerize. Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA reoplication and probably DNA repair. Required for the recruitment of INO80 (and probably the INO80 complex) to sites of DNA damage. Strongly prefer nucleosomes and H3-H4 tetramers over H2A-H2B dimers, suggesting it may act as a nucleosome recognition module within the complex. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 20: |
Line 18: |
| | </div> | | </div> |
| | <div class="pdbe-citations 4fo0" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 4fo0" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Actin-related protein 3D structures|Actin-related protein 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Gerhold, C B]] | + | [[Category: Large Structures]] |
| - | [[Category: Hopfner, K P]] | + | [[Category: Gerhold CB]] |
| - | [[Category: Lakomek, K]] | + | [[Category: Hopfner K-P]] |
| - | [[Category: Seifert, F U]]
| + | [[Category: Lakomek K]] |
| - | [[Category: Actin-related protein]]
| + | [[Category: Seifert FU]] |
| - | [[Category: Chromatin remodeling]] | + | |
| - | [[Category: Gene regulation]] | + | |
| - | [[Category: Nucleosome]]
| + | |
| - | [[Category: Nucleus]]
| + | |
| Structural highlights
Function
ARP8_HUMAN Plays an important role in the functional organization of mitotic chromosomes. Exhibits low basal ATPase activity, and unable to polymerize. Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA reoplication and probably DNA repair. Required for the recruitment of INO80 (and probably the INO80 complex) to sites of DNA damage. Strongly prefer nucleosomes and H3-H4 tetramers over H2A-H2B dimers, suggesting it may act as a nucleosome recognition module within the complex.
Publication Abstract from PubMed
Nuclear actin-related proteins (Arps) are subunits of several chromatin remodelers, but their molecular functions within these complexes are unclear. We report the crystal structure of the INO80 complex subunit Arp8 in its ATP-bound form. Human Arp8 has several insertions in the conserved actin fold that explain its inability to polymerize. Most remarkably, one insertion wraps over the active site cleft and appears to rigidify the domain architecture, while active site features shared with actin suggest an allosterically controlled ATPase activity. Quantitative binding studies with nucleosomes and histone complexes reveal that Arp8 and the Arp8-Arp4-actin-HSA sub-complex of INO80 strongly prefer nucleosomes and H3-H4 tetramers over H2A-H2B dimers, suggesting that Arp8 functions as a nucleosome recognition module. In contrast, Arp4 prefers free (H3-H4)(2) over nucleosomes and may serve remodelers through binding to (dis)assembly intermediates in the remodeling reaction.
Structure of Actin-related protein 8 and its contribution to nucleosome binding.,Gerhold CB, Winkler DD, Lakomek K, Seifert FU, Fenn S, Kessler B, Witte G, Luger K, Hopfner KP Nucleic Acids Res. 2012 Sep 12. PMID:22977180[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Gerhold CB, Winkler DD, Lakomek K, Seifert FU, Fenn S, Kessler B, Witte G, Luger K, Hopfner KP. Structure of Actin-related protein 8 and its contribution to nucleosome binding. Nucleic Acids Res. 2012 Sep 12. PMID:22977180 doi:http://dx.doi.org/10.1093/nar/gks842
|
