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4nsx

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Revision as of 08:38, 18 January 2023

Crystal Structure of the Utp21 tandem WD Domain

Structural highlights

4nsx is a 1 chain structure with sequence from Saccharomyces cerevisiae S288C. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

UTP21_YEAST Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly.^[1]

Publication Abstract from PubMed

Assembly of the eukaryotic ribosome requires a large number of trans-acting proteins and small nucleolar RNAs that transiently associate with the precursor rRNA to facilitate its modification, processing and binding with ribosomal proteins. UTPB is a large evolutionarily conserved complex in the 90S small subunit processome that mediates early processing of 18S rRNA. UTPB consists of six proteins Utp1/Pwp1, Utp6, Utp12/Dip2, Utp13, Utp18 and Utp21 and has abundant WD domains. Here, we determined the crystal structure of the tandem WD domain of yeast Utp21 at 2.1 A resolution, revealing two open-clamshell-shaped beta-propellers. The bottom faces of both WD domains harbor several conserved patches that potentially function as molecular binding sites. We show that residues 100-190 of Utp18 bind to the tandem WD domain of Utp21. Structural mapping of previous crosslinking data shows that the WD domains of Utp18 and Utp1 are organized on two opposite sides of the Utp21 WD domains. This study reports the first structure of a UTPB component and provides insight into the structural organization of the UTPB complex.

Structure of Utp21 Tandem WD Domain Provides Insight into the Organization of the UTPB Complex Involved in Ribosome Synthesis.,Zhang C, Lin J, Liu W, Chen X, Chen R, Ye K PLoS One. 2014 Jan 21;9(1):e86540. doi: 10.1371/journal.pone.0086540. eCollection, 2014 Jan 21. PMID:24466140^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bernstein KA, Gallagher JE, Mitchell BM, Granneman S, Baserga SJ. The small-subunit processome is a ribosome assembly intermediate. Eukaryot Cell. 2004 Dec;3(6):1619-26. PMID:15590835 doi:http://dx.doi.org/10.1128/EC.3.6.1619-1626.2004
↑ Zhang C, Lin J, Liu W, Chen X, Chen R, Ye K. Structure of Utp21 Tandem WD Domain Provides Insight into the Organization of the UTPB Complex Involved in Ribosome Synthesis. PLoS One. 2014 Jan 21;9(1):e86540. doi: 10.1371/journal.pone.0086540. eCollection, 2014 Jan 21. PMID:24466140 doi:http://dx.doi.org/10.1371/journal.pone.0086540

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4nsx"

Categories: Large Structures | Saccharomyces cerevisiae S288C | Ye K | Zhang C

@@ Line 1: / Line 1: @@
 ==Crystal Structure of the Utp21 tandem WD Domain==
-<StructureSection load='4nsx' size='340' side='right' caption='[[4nsx]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
+<StructureSection load='4nsx' size='340' side='right'caption='[[4nsx]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4nsx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NSX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NSX FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4nsx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NSX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NSX FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">L8084.22, UTP21, YLR409C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4nsx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nsx OCA], [https://pdbe.org/4nsx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4nsx RCSB], [https://www.ebi.ac.uk/pdbsum/4nsx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4nsx ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nsx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nsx OCA], [http://pdbe.org/4nsx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4nsx RCSB], [http://www.ebi.ac.uk/pdbsum/4nsx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4nsx ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/UTP21_YEAST UTP21_YEAST]] Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly.<ref>PMID:15590835</ref>
+[https://www.uniprot.org/uniprot/UTP21_YEAST UTP21_YEAST] Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly.<ref>PMID:15590835</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Baker's yeast]]
+[[Category: Large Structures]]
-[[Category: Ye, K]]
+[[Category: Saccharomyces cerevisiae S288C]]
-[[Category: Zhang, C]]
+[[Category: Ye K]]
-[[Category: 90s preribosome]]
+[[Category: Zhang C]]
-[[Category: Protein binding]]
-[[Category: Ribosome biogenesis]]
-[[Category: Small subunit processome]]
-[[Category: Utpb complex]]

4nsx

From Proteopedia

Revision as of 08:38, 18 January 2023

Crystal Structure of the Utp21 tandem WD Domain

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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