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3nvv

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Revision as of 08:08, 4 November 2020

Crystal Structure of Bovine Xanthine Oxidase in Complex with Arsenite

Structural highlights

3nvv is a 6 chain structure with sequence from Bos taurus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , , ,
Related:	3nvw, 3nvy, 3nvz
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[XDH_BOVIN] Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species.

Publication Abstract from PubMed

Xanthine oxidoreductase is a molybdenum-containing enzyme that catalyzes the hydroxylation reaction of sp(2)-hybridized carbon centers of a variety of substrates, including purines, aldehydes, and other heterocyclic compounds. The complex of arsenite-inhibited xanthine oxidase has been characterized previously by UV-vis, electron paramagnetic resonance, and X-ray absorption spectroscopy (XAS), and the catalytically essential sulfido ligand of the square-pyrimidal molybdenum center has been suggested to be involved in arsenite binding through either a mu-sulfido,mu-oxo double bridge or a single mu-sulfido bridge. However, this is contrary to the crystallographically observed single mu-oxo bridge between molybdenum and arsenic in the desulfo form of aldehyde oxidoreductase from Desulfovibrio gigas (an enzyme closely related to xanthine oxidase), whose molybdenum center has an oxo ligand replacing the catalytically essential sulfur, as seen in the functional form of xanthine oxidase. Here we use X-ray crystallography to characterize the molybdenum center of arsenite-inhibited xanthine oxidase and solve the structures of the oxidized and reduced inhibition complexes at 1.82 and 2.11 A resolution, respectively. We observe mu-sulfido,mu-oxo double bridges between molybdenum and arsenic in the active sites of both complexes. Arsenic is four-coordinate with a distorted trigonal-pyramidal geometry in the oxidized complex and three-coordinate with a distorted trigonal-planar geometry in the reduced complex. The doubly bridged binding mode is in agreement with previous XAS data indicating that the catalytically essential sulfur is also essential for the high affinity of reduced xanthine oxidoreductase for arsenite.

X-ray crystal structure of arsenite-inhibited xanthine oxidase: mu-sulfido,mu-oxo double bridge between molybdenum and arsenic in the active site.,Cao H, Hall J, Hille R J Am Chem Soc. 2011 Aug 17;133(32):12414-7. Epub 2011 Jul 21. PMID:21761899^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Cao H, Hall J, Hille R. X-ray crystal structure of arsenite-inhibited xanthine oxidase: mu-sulfido,mu-oxo double bridge between molybdenum and arsenic in the active site. J Am Chem Soc. 2011 Aug 17;133(32):12414-7. Epub 2011 Jul 21. PMID:21761899 doi:10.1021/ja2050265

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3nvv"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of Bovine Xanthine Oxidase in Complex with Arsenite==
-<StructureSection load='3nvv' size='340' side='right' caption='[[3nvv]], [[Resolution|resolution]] 1.82&Aring;' scene=''>
+<StructureSection load='3nvv' size='340' side='right'caption='[[3nvv]], [[Resolution|resolution]] 1.82&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3nvv]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NVV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NVV FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3nvv]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NVV OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3NVV FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AST:ARSENITE'>AST</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=MOS:DIOXOTHIOMOLYBDENUM(VI)+ION'>MOS</scene>, <scene name='pdbligand=MTE:PHOSPHONIC+ACIDMONO-(2-AMINO-5,6-DIMERCAPTO-4-OXO-3,7,8A,9,10,10A-HEXAHYDRO-4H-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-7-YLMETHYL)ESTER'>MTE</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AST:ARSENITE'>AST</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=MOS:DIOXOTHIOMOLYBDENUM(VI)+ION'>MOS</scene>, <scene name='pdbligand=MTE:PHOSPHONIC+ACIDMONO-(2-AMINO-5,6-DIMERCAPTO-4-OXO-3,7,8A,9,10,10A-HEXAHYDRO-4H-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-7-YLMETHYL)ESTER'>MTE</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3nvw|3nvw]], [[3nvy|3nvy]], [[3nvz|3nvz]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nvv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nvv OCA], [http://pdbe.org/3nvv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3nvv RCSB], [http://www.ebi.ac.uk/pdbsum/3nvv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3nvv ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3nvv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nvv OCA], [http://pdbe.org/3nvv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3nvv RCSB], [http://www.ebi.ac.uk/pdbsum/3nvv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3nvv ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 27: / Line 27: @@
 </StructureSection>
 [[Category: Bos taurus]]
+[[Category: Large Structures]]
 [[Category: Cao, H]]
 [[Category: Hille, R]]

3nvv

From Proteopedia

Revision as of 08:08, 4 November 2020

Crystal Structure of Bovine Xanthine Oxidase in Complex with Arsenite

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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