1mxg

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[[Image:1mxg.gif|left|200px]]
[[Image:1mxg.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1mxg |SIZE=350|CAPTION= <scene name='initialview01'>1mxg</scene>, resolution 1.6&Aring;
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The line below this paragraph, containing "STRUCTURE_1mxg", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ACR:ACARBOSE'>ACR</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EOH:ETHANOL'>EOH</scene>, <scene name='pdbligand=ETE:2-{2-[2-2-(METHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHANOL'>ETE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1mxg| PDB=1mxg | SCENE= }}
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|RELATEDENTRY=[[1mwo|1MWO]], [[1mxd|1MXD]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mxg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mxg OCA], [http://www.ebi.ac.uk/pdbsum/1mxg PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1mxg RCSB]</span>
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}}
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'''Crystal Strucutre of a (Ca,Zn)-dependent alpha-amylase from the hyperthermophilic archaeon Pyrococcus woesei in complex with acarbose'''
'''Crystal Strucutre of a (Ca,Zn)-dependent alpha-amylase from the hyperthermophilic archaeon Pyrococcus woesei in complex with acarbose'''
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[[Category: Meyer-Klaucke, W.]]
[[Category: Meyer-Klaucke, W.]]
[[Category: Wilmanns, M.]]
[[Category: Wilmanns, M.]]
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[[Category: (beta/alpha)8-barrel]]
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[[Category: Alpha-amylase]]
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[[Category: alpha-amylase]]
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[[Category: Family 13 glycosyl hydrolase]]
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[[Category: family 13 glycosyl hydrolase]]
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[[Category: Hyperthermostable]]
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[[Category: hyperthermostable]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:50:18 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:22:29 2008''
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Revision as of 22:50, 2 May 2008

Image:1mxg.gif

Template:STRUCTURE 1mxg

Crystal Strucutre of a (Ca,Zn)-dependent alpha-amylase from the hyperthermophilic archaeon Pyrococcus woesei in complex with acarbose


Overview

The crystal structure of the alpha-amylase from the hyperthermophilic archaeon Pyrococcus woesei was solved in the presence of three inhibitors: acarbose, Tris, and zinc. In the absence of exogenous metals, this alpha-amylase bound 1 and 4 molar eq of zinc and calcium, respectively. The structure reveals a novel, activating, two-metal (Ca,Zn)-binding site and a second inhibitory zinc-binding site that is found in the -1 sugar-binding pocket within the active site. The data resolve the apparent paradox between the zinc requirement for catalytic activity and its strong inhibitory effect when added in molar excess. They provide a rationale as to why this alpha-amylase, in contrast to commercially available alpha-amylases, does not require the addition of metal ions for full catalytic activity, suggesting it as an ideal target to maximize the efficiency of industrial processes like liquefaction of starch.

About this Structure

1MXG is a Single protein structure of sequence from Pyrococcus woesei. Full crystallographic information is available from OCA.

Reference

Differential regulation of a hyperthermophilic alpha-amylase with a novel (Ca,Zn) two-metal center by zinc., Linden A, Mayans O, Meyer-Klaucke W, Antranikian G, Wilmanns M, J Biol Chem. 2003 Mar 14;278(11):9875-84. Epub 2002 Dec 12. PMID:12482867 Page seeded by OCA on Sat May 3 01:50:18 2008

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