1n0k

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[[Image:1n0k.gif|left|200px]]
[[Image:1n0k.gif|left|200px]]
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{{Structure
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|PDB= 1n0k |SIZE=350|CAPTION= <scene name='initialview01'>1n0k</scene>
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The line below this paragraph, containing "STRUCTURE_1n0k", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=PGA:2-PHOSPHOGLYCOLIC+ACID'>PGA</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1n0k| PDB=1n0k | SCENE= }}
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|RELATEDENTRY=[[1n0o|1N0O]], [[1g5k|1G5K]], [[1gj1|1GJ1]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n0k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n0k OCA], [http://www.ebi.ac.uk/pdbsum/1n0k PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1n0k RCSB]</span>
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'''NMR Structure of duplex DNA d(CCAAGGXCTTGGG), X is a 3' phosphoglycolate, 5'phosphate gapped lesion'''
'''NMR Structure of duplex DNA d(CCAAGGXCTTGGG), X is a 3' phosphoglycolate, 5'phosphate gapped lesion'''
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==About this Structure==
==About this Structure==
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1N0K is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N0K OCA].
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N0K OCA].
==Reference==
==Reference==
Synthesis, characterization and solution structure of tethered oligonucleotides containing an internal 3'-phosphoglycolate, 5'-phosphate gapped lesion., Junker HD, Hoehn ST, Bunt RC, Marathius V, Chen J, Turner CJ, Stubbe J, Nucleic Acids Res. 2002 Dec 15;30(24):5497-508. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12490718 12490718]
Synthesis, characterization and solution structure of tethered oligonucleotides containing an internal 3'-phosphoglycolate, 5'-phosphate gapped lesion., Junker HD, Hoehn ST, Bunt RC, Marathius V, Chen J, Turner CJ, Stubbe J, Nucleic Acids Res. 2002 Dec 15;30(24):5497-508. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12490718 12490718]
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[[Category: Protein complex]]
 
[[Category: Bunt, R C.]]
[[Category: Bunt, R C.]]
[[Category: Chen, J.]]
[[Category: Chen, J.]]
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[[Category: Stubbe, J.]]
[[Category: Stubbe, J.]]
[[Category: Turner, C J.]]
[[Category: Turner, C J.]]
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[[Category: bleomycin]]
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[[Category: Bleomycin]]
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[[Category: dna]]
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[[Category: Dna]]
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[[Category: nmr]]
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[[Category: Nmr]]
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[[Category: phosphoglycolate,]]
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[[Category: Phosphoglycolate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:56:23 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:23:40 2008''
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Revision as of 22:56, 2 May 2008

Image:1n0k.gif

Template:STRUCTURE 1n0k

NMR Structure of duplex DNA d(CCAAGGXCTTGGG), X is a 3' phosphoglycolate, 5'phosphate gapped lesion


Overview

Bleomycins (BLMs) are antitumor antibiotics that in the presence of iron and oxygen mediate DNA damage by 4'-hydrogen atom abstraction of pyrimidines 3' to guanines. The resulting 4'-deoxyribose radicals can be trapped by O2 and ultimately result in the formation of base-propenal and gapped DNA with 3'-phosphoglycolate (3'-PG) and 5'-phosphate (5'-P) ends. The role of this lesion in triggering double-strand cleavage of duplex DNA by a single BLM molecule and the mechanism by which this lesion is repaired in vivo remain unsolved problems. The structure of these lesions is an essential step in addressing both of these problems. Duplex DNAs (13mers containing tethered hexaethylene glycol linkers) with GTAC and GGCC cleavage sites have been synthesized in which gaps containing 3'-PG and 5'-P ends at the sites of BLM cleavage have been inserted. The former sequence represents a hot spot for double-strand cleavage, while the latter is a hot spot for single-strand cleavage. Analytical methods to characterize the lesioned products have been developed. These oligonucleotides have been examined using 2D NMR methods and molecular modeling. The studies reveal that the lesioned DNAs are B-form and the 3'-PG and 5'-P are extrahelical. The base opposite the gap and the base pairs adjacent to the gap remain well stacked in the DNA duplex. Titrations of the lesioned GGCC oligomer with HOO-CoBLM leads to a mixture of complexes, in contrast to results of a similar titration with the lesioned GTAC oligomer.

About this Structure

Full crystallographic information is available from OCA.

Reference

Synthesis, characterization and solution structure of tethered oligonucleotides containing an internal 3'-phosphoglycolate, 5'-phosphate gapped lesion., Junker HD, Hoehn ST, Bunt RC, Marathius V, Chen J, Turner CJ, Stubbe J, Nucleic Acids Res. 2002 Dec 15;30(24):5497-508. PMID:12490718 Page seeded by OCA on Sat May 3 01:56:23 2008

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