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| | ==Human Cytochrome P450 1A1 in complex with alpha-naphthoflavone== | | ==Human Cytochrome P450 1A1 in complex with alpha-naphthoflavone== |
| - | <StructureSection load='4i8v' size='340' side='right' caption='[[4i8v]], [[Resolution|resolution]] 2.60Å' scene=''> | + | <StructureSection load='4i8v' size='340' side='right'caption='[[4i8v]], [[Resolution|resolution]] 2.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4i8v]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4I8V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4I8V FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4i8v]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4I8V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4I8V FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BHF:2-PHENYL-4H-BENZO[H]CHROMEN-4-ONE'>BHF</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BHF:2-PHENYL-4H-BENZO[H]CHROMEN-4-ONE'>BHF</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CYP1A1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4i8v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4i8v OCA], [https://pdbe.org/4i8v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4i8v RCSB], [https://www.ebi.ac.uk/pdbsum/4i8v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4i8v ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Unspecific_monooxygenase Unspecific monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.1 1.14.14.1] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4i8v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4i8v OCA], [http://pdbe.org/4i8v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4i8v RCSB], [http://www.ebi.ac.uk/pdbsum/4i8v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4i8v ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CP1A1_HUMAN CP1A1_HUMAN]] Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. | + | [https://www.uniprot.org/uniprot/CP1A1_HUMAN CP1A1_HUMAN] Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[Cytochrome P450|Cytochrome P450]] | + | *[[Cytochrome P450 3D structures|Cytochrome P450 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Unspecific monooxygenase]] | + | [[Category: Large Structures]] |
| - | [[Category: Scott, E E]] | + | [[Category: Scott EE]] |
| - | [[Category: Walsh, A A]] | + | [[Category: Walsh AA]] |
| - | [[Category: Aryl hydrocarbon hydroxylase]]
| + | |
| - | [[Category: Cyp1a1]]
| + | |
| - | [[Category: Cytochrome p450]]
| + | |
| - | [[Category: Heme]]
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| - | [[Category: Monooxygenase]]
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| - | [[Category: Oxidoreductase]]
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| - | [[Category: P450]]
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| - | [[Category: P450 1a1]]
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| Structural highlights
Function
CP1A1_HUMAN Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Publication Abstract from PubMed
Cytochrome P450 (CYP) 1A1 is an extrahepatic monooxygenase involved in the metabolism of endogenous substrates and drugs, as well as the activation of certain toxins and environmental pollutants. CYP1A1 is particularly well known for its ability to biotransform polycyclic aromatic hydrocarbons (PAHs), such as benzo[a]pyrene in tobacco smoke, into carcinogens. CYP1A1 possesses functional similarities and differences with human CYP1A2 and CYP1B1 enzymes, but the structural basis for this has been unclear. We determined a 2.6 A structure of human CYP1A1 with the inhibitor alpha-naphthoflavone (ANF). ANF binds within an enclosed active site, with the planar benzochromen-4-one core packed flat against the I helix that composes one wall of the active site, and the 2-phenyl substituent oriented toward the catalytic heme iron. Comparisons with previously determined structures of the related cytochrome P450 1A2 and 1B1 enzymes reveal distinct features among the active sites that may underlie the functional variability of these enzymes. Finally, docking studies probed the ability of CYP1A structures to assist in understanding their known in vitro interactions with several typical substrates and inhibitors.
Human Cytochrome P450 1A1 Structure and Utility In Understanding Drug and Xenobiotic Metabolism.,Walsh AA, Szklarz GD, Scott EE J Biol Chem. 2013 Mar 18. PMID:23508959[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Walsh AA, Szklarz GD, Scott EE. Human Cytochrome P450 1A1 Structure and Utility In Understanding Drug and Xenobiotic Metabolism. J Biol Chem. 2013 Mar 18. PMID:23508959 doi:10.1074/jbc.M113.452953
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