This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

4ght

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 07:39, 27 March 2019

Crystal structure of EV71 3C proteinase in complex with AG7088

Structural highlights

4ght is a 2 chain structure with sequence from Ev-71. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	4ghq, 4ghv, 4ghx, 4ghy, 4ghz
Activity:	Picornain 3C, with EC number 3.4.22.28
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[A9XG43_9ENTO] Protein 2C associates with and induces structural rearrangements of intracellular membranes. It displays RNA-binding, nucleotide binding and NTPase activities (By similarity).[SAAS:SAAS000199_004_016611] Protein 3C is a cysteine protease that generates mature viral proteins from the precursor polyprotein. In addition to its proteolytic activity, it binds to viral RNA, and thus influences viral genome replication. RNA and substrate bind co-operatively to the protease (By similarity).[SAAS:SAAS000199_004_042266] RNA-directed RNA polymerase 3D-POL replicates genomic and antigenomic RNA by recognizing replications specific signals (By similarity).[SAAS:SAAS000199_004_010047]

Publication Abstract from PubMed

The crystal structure of 3C proteinase (3C(pro)) from Enterovirus 71 (EV71) was determined in space group C2221 to 2.2 A resolution. The fold was similar to that of 3C(pro) from other picornaviruses, but the difference in the beta-ribbon reported in a previous structure was not observed. This beta-ribbon was folded over the substrate-binding cleft and constituted part of the essential binding sites for interaction with the substrate. The structure of its complex with rupintrivir (AG7088), a peptidomimetic inhibitor, was also characterized in space group P212121 to 1.96 A resolution. The inhibitor was accommodated without any spatial hindrance despite the more constricted binding site; this was confirmed by functional assays, in which the inhibitor showed comparable potency towards EV71 3C(pro) and human rhinovirus 3C(pro), which is the target that rupintrivir was designed against.

Structures of Enterovirus 71 3C proteinase (strain E2004104-TW-CDC) and its complex with rupintrivir.,Wu C, Cai Q, Chen C, Li N, Peng X, Cai Y, Yin K, Chen X, Wang X, Zhang R, Liu L, Chen S, Li J, Lin T Acta Crystallogr D Biol Crystallogr. 2013 May;69(Pt 5):866-71. doi:, 10.1107/S0907444913002862. Epub 2013 Apr 19. PMID:23633597^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wu C, Cai Q, Chen C, Li N, Peng X, Cai Y, Yin K, Chen X, Wang X, Zhang R, Liu L, Chen S, Li J, Lin T. Structures of Enterovirus 71 3C proteinase (strain E2004104-TW-CDC) and its complex with rupintrivir. Acta Crystallogr D Biol Crystallogr. 2013 May;69(Pt 5):866-71. doi:, 10.1107/S0907444913002862. Epub 2013 Apr 19. PMID:23633597 doi:10.1107/S0907444913002862

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4ght"

@@ Line 1: / Line 1: @@
 ==Crystal structure of EV71 3C proteinase in complex with AG7088==
-<StructureSection load='4ght' size='340' side='right' caption='[[4ght]], [[Resolution|resolution]] 1.96&Aring;' scene=''>
+<StructureSection load='4ght' size='340' side='right'caption='[[4ght]], [[Resolution|resolution]] 1.96&Aring;' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[4ght]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ev-71 Ev-71]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GHT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GHT FirstGlance]. <br>
@@ Line 28: / Line 28: @@
 </StructureSection>
 [[Category: Ev-71]]
+[[Category: Large Structures]]
 [[Category: Picornain 3C]]
 [[Category: Cai, Q]]

4ght

From Proteopedia

Revision as of 07:39, 27 March 2019

Crystal structure of EV71 3C proteinase in complex with AG7088

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox