1n3b

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[[Image:1n3b.gif|left|200px]]
[[Image:1n3b.gif|left|200px]]
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{{Structure
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|PDB= 1n3b |SIZE=350|CAPTION= <scene name='initialview01'>1n3b</scene>, resolution 1.8&Aring;
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The line below this paragraph, containing "STRUCTURE_1n3b", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Dephospho-CoA_kinase Dephospho-CoA kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.24 2.7.1.24] </span>
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|GENE= COAE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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{{STRUCTURE_1n3b| PDB=1n3b | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n3b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n3b OCA], [http://www.ebi.ac.uk/pdbsum/1n3b PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1n3b RCSB]</span>
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'''Crystal Structure of Dephosphocoenzyme A kinase from Escherichia coli'''
'''Crystal Structure of Dephosphocoenzyme A kinase from Escherichia coli'''
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[[Category: Matte, A.]]
[[Category: Matte, A.]]
[[Category: Toole, N O.]]
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[[Category: Alpha/beta]]
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[[Category: bsgi]]
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[[Category: Bsgi]]
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[[Category: montreal-kingston bacterial structural genomics initiative]]
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[[Category: Montreal-kingston bacterial structural genomics initiative]]
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[[Category: p-loop]]
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[[Category: P-loop]]
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[[Category: structural genomic]]
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[[Category: Structural genomic]]
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[[Category: trimer]]
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[[Category: Trimer]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:02:20 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:24:45 2008''
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Revision as of 23:02, 2 May 2008

Image:1n3b.gif

Template:STRUCTURE 1n3b

Crystal Structure of Dephosphocoenzyme A kinase from Escherichia coli


Overview

Coenzyme A (CoA) is an essential cofactor used in a wide variety of biochemical pathways. The final step in the biosynthesis of CoA is catalyzed by dephosphocoenzyme A kinase (DPCK, E.C. 2.7.1.24). Here we report the crystal structure of DPCK from Escherichia coli at 1.8 A resolution. This enzyme forms a tightly packed trimer in its crystal state, in contrast to its observed monomeric structure in solution and to the monomeric, homologous DPCK structure from Haemophilus influenzae. We have confirmed the existence of the trimeric form of the enzyme in solution using gel filtration chromatography measurements. Dephospho-CoA kinase is structurally similar to many nucleoside kinases and other P-loop-containing nucleotide triphosphate hydrolases, despite having negligible sequence similarity to these enzymes. Each monomer consists of five parallel beta-strands flanked by alpha-helices, with an ATP-binding site formed by a P-loop motif. Orthologs of the E. coli DPCK sequence exist in a wide range of organisms, including humans. Multiple alignment of orthologous DPCK sequences reveals a set of highly conserved residues in the vicinity of the nucleotide/CoA binding site.

About this Structure

1N3B is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure of a trimeric form of dephosphocoenzyme A kinase from Escherichia coli., O'Toole N, Barbosa JA, Li Y, Hung LW, Matte A, Cygler M, Protein Sci. 2003 Feb;12(2):327-36. PMID:12538896 Page seeded by OCA on Sat May 3 02:02:20 2008

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