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| | ==Crystal structure of small single-stranded DNA-binding protein from Streptomyces coelicolor== | | ==Crystal structure of small single-stranded DNA-binding protein from Streptomyces coelicolor== |
| - | <StructureSection load='4dam' size='340' side='right' caption='[[4dam]], [[Resolution|resolution]] 1.70Å' scene=''> | + | <StructureSection load='4dam' size='340' side='right'caption='[[4dam]], [[Resolution|resolution]] 1.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4dam]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_coelicolor"_(muller_1908)_lieske_1921 "actinomyces coelicolor" (muller 1908) lieske 1921]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DAM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DAM FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4dam]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DAM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DAM FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3eiv|3eiv]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dam OCA], [https://pdbe.org/4dam PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dam RCSB], [https://www.ebi.ac.uk/pdbsum/4dam PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dam ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SCC61A.04c, SCO2683, ssb1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 "Actinomyces coelicolor" (Muller 1908) Lieske 1921])</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dam OCA], [http://pdbe.org/4dam PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4dam RCSB], [http://www.ebi.ac.uk/pdbsum/4dam PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4dam ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SSB1_STRCO SSB1_STRCO]] This protein is essential for replication of the chromosome. It is also involved in DNA recombination and repair (By similarity). | + | [[https://www.uniprot.org/uniprot/SSB1_STRCO SSB1_STRCO]] This protein is essential for replication of the chromosome. It is also involved in DNA recombination and repair (By similarity). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[Single-stranded DNA-binding protein|Single-stranded DNA-binding protein]] | + | *[[Single-stranded DNA-binding protein 3D structures|Single-stranded DNA-binding protein 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Filic, Z]] | + | [[Category: Large Structures]] |
| - | [[Category: Herron, P]] | + | [[Category: Streptomyces coelicolor]] |
| - | [[Category: Ivic, N]] | + | [[Category: Filic Z]] |
| - | [[Category: Luic, M]] | + | [[Category: Herron P]] |
| - | [[Category: Manjasetty, B A]] | + | [[Category: Ivic N]] |
| - | [[Category: Paradzik, T]] | + | [[Category: Luic M]] |
| - | [[Category: Vujaklija, D]] | + | [[Category: Manjasetty BA]] |
| - | [[Category: Dna binding protein]] | + | [[Category: Paradzik T]] |
| - | [[Category: Dna-binding]] | + | [[Category: Vujaklija D]] |
| - | [[Category: Ob-fold]]
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| - | [[Category: Single-stranded dna]]
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| Structural highlights
Function
[SSB1_STRCO] This protein is essential for replication of the chromosome. It is also involved in DNA recombination and repair (By similarity).
Publication Abstract from PubMed
The linear chromosome of Streptomyces coelicolor contains two paralogous ssb genes, ssbA and ssbB. Following mutational analysis, we concluded that ssbA is essential, whereas ssbB plays a key role in chromosome segregation during sporulation. In the ssbB mutant, approximately 30% of spores lacked DNA. The two ssb genes were expressed differently; in minimal medium, gene expression was prolonged for both genes and significantly upregulated for ssbB. The ssbA gene is transcribed as part of a polycistronic mRNA from two initiation sites, 163 bp and 75 bp upstream of the rpsF translational start codon. The ssbB gene is transcribed as a monocistronic mRNA, from an unusual promoter region, 73 bp upstream of the AUG codon. Distinctive DNA-binding affinities of single-stranded DNA-binding proteins monitored by tryptophan fluorescent quenching and electrophoretic mobility shift were observed. The crystal structure of SsbB at 1.7 A resolution revealed a common OB-fold, lack of the clamp-like structure conserved in SsbA and previously unpublished S-S bridges between the A/B and C/D subunits. This is the first report of the determination of paralogous single-stranded DNA-binding protein structures from the same organism. Phylogenetic analysis revealed frequent duplication of ssb genes in Actinobacteria, whereas their strong retention suggests that they are involved in important cellular functions.
Structure-function relationships of two paralogous single-stranded DNA-binding proteins from Streptomyces coelicolor: implication of SsbB in chromosome segregation during sporulation.,Paradzik T, Ivic N, Filic Z, Manjasetty BA, Herron P, Luic M, Vujaklija D Nucleic Acids Res. 2013 Apr 1;41(6):3659-72. doi: 10.1093/nar/gkt050. Epub 2013, Feb 7. PMID:23393191[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Paradzik T, Ivic N, Filic Z, Manjasetty BA, Herron P, Luic M, Vujaklija D. Structure-function relationships of two paralogous single-stranded DNA-binding proteins from Streptomyces coelicolor: implication of SsbB in chromosome segregation during sporulation. Nucleic Acids Res. 2013 Apr 1;41(6):3659-72. doi: 10.1093/nar/gkt050. Epub 2013, Feb 7. PMID:23393191 doi:http://dx.doi.org/10.1093/nar/gkt050
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