5ibw

Publication Abstract from PubMed

Myosin light chains are key regulators of class 1 myosins and typically comprise two domains, with calmodulin being the archetypal example. They bind IQ motifs within the myosin neck region and amplify conformational changes in motor domain. A single lobe light chain, myosin light chain C (MlcC), was recently identified and shown to specifically bind to two sequentially divergent IQ motifs of the Dictyostelium myosin-1C. Towards providing a molecular basis of this interaction, the structures of apo-MlcC and a 2:1 MlcC:myosin-1C neck complex were determined. The two non-functional EF-hand motifs of MlcC pack together to form a globular four-helix bundle that opens up to expose a central hydrophobic groove, which interacts the N-terminal portion of the divergent IQ1 and IQ2 motifs. N- and C-terminal regions of MlcC make critical contacts that contribute to its specific interactions with the myosin-1C divergent IQ motifs; contacts that deviate from the traditional mode of calmodulin-IQ recognition.

Structure of the single-lobe myosin light chain C in complex with the light chain-binding domains of myosin-1C provides insights into divergent IQ-motif recognition.,Langelaan DN, Liburd J, Yang Y, Miller E, Chitayat S, Crawley SW, Cote GP, Smith SP J Biol Chem. 2016 Jul 27. pii: jbc.M116.746313. PMID:27466369^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.