1n87
From Proteopedia
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'''Solution structure of the U-box of Prp19''' | '''Solution structure of the U-box of Prp19''' | ||
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[[Category: Kooi, C W.Vander.]] | [[Category: Kooi, C W.Vander.]] | ||
[[Category: Ohi, M D.]] | [[Category: Ohi, M D.]] | ||
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| - | [[Category: | + | [[Category: U-box]] |
| - | [[Category: | + | [[Category: Ubiquitin ligase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:13:01 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 23:13, 2 May 2008
Solution structure of the U-box of Prp19
Overview
The structure of the U-box in the essential Saccharomyces cerevisiae pre-mRNA splicing factor Prp19p has been determined by NMR. The conserved zinc-binding sites supporting the cross-brace arrangement in RING-finger domains are replaced by hydrogen-bonding networks in the U-box. These hydrogen-bonding networks are necessary for the structural stabilization and activity of the U-box. A conservative Val-->Ile point mutation in the Prp19p U-box domain leads to pre-mRNA splicing defects in vivo. NMR analysis of this mutant shows that the substitution disrupts structural integrity of the U-box domain. Furthermore, comparison of the Prp19p U-box domain with known RING-E2 complex structures demonstrates that both U-box and RING-fingers contain a conserved interaction surface. Mutagenesis of residues at this interface, while not perturbing the structure of the U-box, abrogates Prp19p function in vivo. These comparative structural and functional analyses imply that the U-box and its associated ubiquitin ligase activity are critical for Prp19p function in vivo.
About this Structure
1N87 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structural insights into the U-box, a domain associated with multi-ubiquitination., Ohi MD, Vander Kooi CW, Rosenberg JA, Chazin WJ, Gould KL, Nat Struct Biol. 2003 Apr;10(4):250-5. PMID:12627222 Page seeded by OCA on Sat May 3 02:13:01 2008
