5gp1
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of ZIKV NS5 Methyltransferase in complex with GTP and SAH== | |
| + | <StructureSection load='5gp1' size='340' side='right' caption='[[5gp1]], [[Resolution|resolution]] 2.44Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5gp1]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GP1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GP1 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTA:P1-7-METHYLGUANOSINE-P3-ADENOSINE-5,5-TRIPHOSPHATE'>GTA</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5goz|5goz]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5gp1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gp1 OCA], [http://pdbe.org/5gp1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gp1 RCSB], [http://www.ebi.ac.uk/pdbsum/5gp1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gp1 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Recent outbreak of flavivirus Zika virus (ZIKV) in America has urged the basic as well as translational studies of this important human pathogen. The nonstructural protein 5 (NS5) of the flavivirus has an N-terminal methyltransferase (MTase) domain that plays critical roles in viral RNA genome capping. The null mutant of NS5 MTase is lethal for virus. Therefore, NS5 is a potential drug target for the treatment of Zika virus infection. In this study, we determined crystal structures of the ZIKV MTase in complex with GTP and RNA cap analogue 7meGpppA. Structural analyses revealed highly conserved GTP/cap-binding pocket and S-adenosylmethionine (SAM)-binding pocket. Two conformations of the second base of the cap were identified, which suggests the flexibility of RNA conformation. In addition, the ligand-binding pockets identified a continuous region of hotspots suitable for drug design. Docking calculation shows that the Dengue virus inhibitor compound 10 may bind to the ZIKV MTase. | ||
| - | + | Structure of the NS5 methyltransferase from Zika virus and implications in inhibitor design.,Zhang C, Feng T, Cheng J, Li Y, Yin X, Zeng W, Jin X, Li Y, Guo F, Jin T Biochem Biophys Res Commun. 2016 Nov 17. pii: S0006-291X(16)31963-5. doi:, 10.1016/j.bbrc.2016.11.098. PMID:27866982<ref>PMID:27866982</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| + | <div class="pdbe-citations 5gp1" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Jin, T]] | [[Category: Jin, T]] | ||
[[Category: Zhang, C]] | [[Category: Zhang, C]] | ||
| + | [[Category: Methyltransferase gtp complex]] | ||
| + | [[Category: Transferase]] | ||
Revision as of 19:37, 9 December 2016
Crystal structure of ZIKV NS5 Methyltransferase in complex with GTP and SAH
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