This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
5kyo
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Crystal Structure of CYP101J2== | |
| + | <StructureSection load='5kyo' size='340' side='right' caption='[[5kyo]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5kyo]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KYO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KYO FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kyo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kyo OCA], [http://pdbe.org/5kyo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kyo RCSB], [http://www.ebi.ac.uk/pdbsum/5kyo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kyo ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The cytochrome P450 monooxygenases (P450s) catalyse a vast array of oxygenation reactions that can be useful in biocatalytic applications. CYP101J2 from Sphingobium yanoikuyae is a P450 that catalyses the hydroxylation of 1,8-cineole. Here we report the crystallisation and X-ray structure elucidation of recombinant CYP101J2 to 1.8 A resolution. The CYP101J2 structure shows the canonical P450-fold and has an open conformation in the absence of substrate. Analysis of the structure revealed that CYP101J2, in the absence of substrate, forms a well-ordered substrate-binding channel that suggests a unique form of substrate guidance in comparison to other bacterial 1,8-cineole-hydroxylating P450 enzymes. This article is protected by copyright. All rights reserved. | ||
| - | + | X-ray crystal structure of cytochrome P450 monooxygenase CYP101J2 from Sphingobium yanoikuyae strain B2.,Unterweger B, Drinkwater N, Johanesen P, Lyras D, Dumsday GJ, McGowan S Proteins. 2016 Dec 9. doi: 10.1002/prot.25227. PMID:27936485<ref>PMID:27936485</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 5kyo" style="background-color:#fffaf0;"></div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Drinkwater, N]] | [[Category: Drinkwater, N]] | ||
| + | [[Category: Dumsday, G J]] | ||
| + | [[Category: McGowan, S]] | ||
[[Category: Unterweger, B]] | [[Category: Unterweger, B]] | ||
| - | [[Category: | + | [[Category: 8-cineole hydroxylation]] |
| + | [[Category: Cytochrome p450 monooxygenase]] | ||
| + | [[Category: Oxidoreductase]] | ||
Revision as of 16:38, 4 January 2017
Crystal Structure of CYP101J2
| |||||||||||
