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4pk9

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Revision as of 12:01, 6 November 2017

The Crystal Structure of Native Patatin

Structural highlights

4pk9 is a 1 chain structure with sequence from Heartleaf nightshade. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	4pka, 1oxw, 4pkb
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Patatin is a non-specific plant lipase and the eponymous member of a broad class of serine hydrolases termed the patatin-like phospholipase domain containing proteins (PNPLAs). Certain PNPLA family members can be inhibited by organophosphorus (OP) compounds. Currently, no structural data are available on the modes of interaction between the PNPLAs and OP compounds or their native substrates. To this end, we present the crystal structure of patatin-17 (pat17) in its native state as well as following inhibition with methyl arachidonyl fluorophosphonate (MAFP) and inhibition/aging with diisopropylphosphorofluoridate (DFP). The native pat17 structure revealed the existence of two portals (portal1 and portal2) that lead to its active-site chamber. The DFP-inhibited enzyme underwent the aging process with the negatively charged phosphoryl oxygen, resulting from the loss of an isopropyl group, being within hydrogen-binding distance to the oxyanion hole. The MAFP-inhibited pat17 structure showed that MAFP did not age following its interaction with the nucleophilic serine residue (Ser77) of pat17 since its O-methyl group was intact. The MAFP moiety is oriented with its phosphoryl oxygen in close proximity to the oxyanion hole of pat17 and its O-methyl group located farther away from the oxyanion hole of pat17 relative to the DFP-bound state. The orientation of the alkoxy oxygens within the two OP compounds suggests a role for the oxyanion hole in stabilizing the emerging negative charge on the oxygen during the aging reaction. The arachidonic acid side chain of MAFP could be contained within portals 1 or 2. Comparisons of pat17 in the native, inhibited, and aged states showed no significant global conformational changes with respect to their Calpha backbones, consistent with observations from other alpha/beta hydrolases such as group VIIA phospholipase A2.

Crystal structure of patatin-17 in complex with aged and non-aged organophosphorus compounds.,Wijeyesakere SJ, Richardson RJ, Stuckey JA PLoS One. 2014 Sep 23;9(9):e108245. doi: 10.1371/journal.pone.0108245., eCollection 2014. PMID:25248161^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wijeyesakere SJ, Richardson RJ, Stuckey JA. Crystal structure of patatin-17 in complex with aged and non-aged organophosphorus compounds. PLoS One. 2014 Sep 23;9(9):e108245. doi: 10.1371/journal.pone.0108245., eCollection 2014. PMID:25248161 doi:http://dx.doi.org/10.1371/journal.pone.0108245

1 Structural highlights
2 Publication Abstract from PubMed
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4pk9"

@@ Line 3: / Line 3: @@
 <StructureSection load='4pk9' size='340' side='right' caption='[[4pk9]], [[Resolution|resolution]] 1.96&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4pk9]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PK9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PK9 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4pk9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Heartleaf_nightshade Heartleaf nightshade]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PK9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PK9 FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4pka|4pka]], [[1oxw|1oxw]], [[4pkb|4pkb]]</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pk9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pk9 OCA], [http://pdbe.org/4pk9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pk9 RCSB], [http://www.ebi.ac.uk/pdbsum/4pk9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pk9 ProSAT]</span></td></tr>
@@ Line 20: / Line 20: @@
 __TOC__
 </StructureSection>
+[[Category: Heartleaf nightshade]]
 [[Category: Richardson, R J]]
 [[Category: Stuckey, J A]]

4pk9

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Revision as of 12:01, 6 November 2017

The Crystal Structure of Native Patatin

Structural highlights

Publication Abstract from PubMed

References

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