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Publication Abstract from PubMed

Superoxide reductases (SORs) are metalloproteins which constitute the most recently identified oxygen-detoxification system in anaerobic and microaerobic bacteria and archaea. SORs are involved in scavenging superoxide radicals from the cell by catalyzing the reduction of superoxide ({\rm O}_{2};{\bullet -}) to hydrogen peroxide and are characterized by a catalytic nonhaem iron centre coordinated by four histidine ligands and one cysteine ligand. Ignicoccus hospitalis, a hyperthermophilic crenarchaeon, is known to have a neelaredoxin-type SOR that keeps toxic oxygen species levels under control. Blue crystals of recombinant I. hospitalis oxidized neelaredoxin (14.1 kDa, 124 residues) were obtained. These crystals diffracted to 2.4 A resolution in-house at room temperature and belonged to the hexagonal space group P6(2)22 or P6(4)22, with unit-cell parameters a = b = 108, c = 64 A. Cell-content analysis indicated the presence of one monomer in the asymmetric unit.

Cloning, purification, crystallization and X-ray crystallographic analysis of Ignicoccus hospitalis neelaredoxin.,Pinho FG, Romao CV, Pinto AF, Saraiva LM, Huber H, Matias PM, Teixeira M, Bandeiras TM Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1;66(Pt 5):605-7. doi:, 10.1107/S1744309110012030. Epub 2010 Apr 30. PMID:20445270^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.