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| | ==Adhiron: a stable and versatile peptide display scaffold - full length adhiron== | | ==Adhiron: a stable and versatile peptide display scaffold - full length adhiron== |
| - | <StructureSection load='4n6t' size='340' side='right' caption='[[4n6t]], [[Resolution|resolution]] 1.75Å' scene=''> | + | <StructureSection load='4n6t' size='340' side='right'caption='[[4n6t]], [[Resolution|resolution]] 1.75Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4n6t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N6T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4N6T FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4n6t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N6T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N6T FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4n6u|4n6u]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n6t OCA], [https://pdbe.org/4n6t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n6t RCSB], [https://www.ebi.ac.uk/pdbsum/4n6t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n6t ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PHYTOCYSTATIN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=32630 SYNTHETIC CONSTRUCT sequences])</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n6t OCA], [http://pdbe.org/4n6t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4n6t RCSB], [http://www.ebi.ac.uk/pdbsum/4n6t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4n6t ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Synthetic construct sequences]] | + | [[Category: Large Structures]] |
| - | [[Category: Mcpherson, M]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Nettleship, J E]] | + | [[Category: Mcpherson M]] |
| - | [[Category: Owen, R L]] | + | [[Category: Nettleship JE]] |
| - | [[Category: Owens, R J]] | + | [[Category: Owen RL]] |
| - | [[Category: Tomlinson, D]] | + | [[Category: Owens RJ]] |
| - | [[Category: Consensus protein]]
| + | [[Category: Tomlinson D]] |
| - | [[Category: De novo protein]]
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| - | [[Category: Protein scaffold]]
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| Structural highlights
Publication Abstract from PubMed
We have designed a novel non-antibody scaffold protein, termed Adhiron, based on a phytocystatin consensus sequence. The Adhiron scaffold shows high thermal stability (Tm ca. 101 degrees C), and is expressed well in Escherichia coli. We have determined the X-ray crystal structure of the Adhiron scaffold to 1.75 A resolution revealing a compact cystatin-like fold. We have constructed a phage-display library in this scaffold by insertion of two variable peptide regions. The library is of high quality and complexity comprising 1.3 x 1010 clones. To demonstrate library efficacy, we screened against the yeast Small Ubiquitin-like Modifier (SUMO). In selected clones, variable region 1 often contained sequences homologous to the known SUMO interactive motif (V/I-X-V/I-V/I). Four Adhirons were further characterised and displayed low nanomolar affinities and high specificity for yeast SUMO with essentially no cross-reactivity to human SUMO protein isoforms. We have identified binders against >100 target molecules to date including as examples, a fibroblast growth factor (FGF1), platelet endothelial cell adhesion molecule (PECAM-1; CD31), the SH2 domain Grb2 and a 12-aa peptide. Adhirons are highly stable and well expressed allowing highly specific binding reagents to be selected for use in molecular recognition applications.
Adhiron: a stable and versatile peptide display scaffold for molecular recognition applications.,Tiede C, Tang AA, Deacon SE, Mandal U, Nettleship JE, Owen RL, George SE, Harrison DJ, Owens RJ, Tomlinson DC, McPherson MJ Protein Eng Des Sel. 2014 Mar 25. PMID:24668773[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Tiede C, Tang AA, Deacon SE, Mandal U, Nettleship JE, Owen RL, George SE, Harrison DJ, Owens RJ, Tomlinson DC, McPherson MJ. Adhiron: a stable and versatile peptide display scaffold for molecular recognition applications. Protein Eng Des Sel. 2014 Mar 25. PMID:24668773 doi:http://dx.doi.org/10.1093/protein/gzu007
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